Abstract：
In this study, oversized microplastics (OMPs) were intentionally introduced into soil containing manure-borne doxycycline (DOX). This strategic approach was used to systematically examine the effects of combined OMP and DOX pollution on the growth of pak choi, analyze alterations in soil environmental metabolites, and explore the potential migration of antibiotic resistance genes (ARGs). The results revealed a more pronounced impact of DOX than of OMPs. Slender-fiber OMPs (SF OMPs) had a more substantial influence on the growth of pak choi than did coarse-fiber OMPs (CF OMPs). Conversely, CF OMPs had a more significant effect on the migration of ARGs within the system. When DOX was combined with OMPs, the negative effects of DOX on pak choi growth were mitigated through the synthesis of indole through the adjustment of carbon metabolism and amino acid metabolism in pak choi roots. In this process, Pseudohongiellaceae and Xanthomonadaceae were key bacteria. During the migration of ARGs, the potential host bacterium Limnobacter should be considered. Additionally, the majority of potential host bacteria in the pak choi endophytic environment were associated with tetG. This study provides insights into the intricate interplay among DOX, OMPs, ARGs, plant growth, soil metabolism, and the microbiome.
摘要：
在这项研究中,故意将过大的微塑料（OMPs）引入含有粪便携带的强力霉素（DOX）的土壤中。此战略方法用于系统地检查OMP和DOX污染对小白菜生长的影响，分析土壤环境代谢物的变化，并探索抗生素抗性基因(ARGs)的潜在迁移。结果表明，DOX的影响比OMP的影响更明显。与粗纤维OMPs（CFOMPs）相比，细长纤维OMPs（SFOMPs）对小白菜生长的影响更大。相反，CFOMPs对系统内ARGs迁移的影响更为显著。当DOX与OMP结合时，通过调节小白菜根中的碳代谢和氨基酸代谢，通过吲哚的合成减轻了DOX对小白菜生长的负面影响。在这个过程中,假参科和黄参科是关键细菌。在ARG的迁移过程中，应考虑潜在的宿主细菌。此外,pakchoi内生环境中的大多数潜在宿主细菌与tetG相关。这项研究提供了对DOX之间复杂相互作用的见解，OMPs,ARGs,植物生长,土壤代谢，和微生物组。
