PDF(Pubmed)
Abstract：
UNASSIGNED: Little is known about the proteomic changes at the portals of entry in rainbow trout after infection with the myxozoan parasites, Myxobolus cerebralis, and Tetracapsuloides bryosalmonae. Whirling disease (WD) is a severe disease of salmonids, caused by the myxosporean M. cerebralis, while, proliferative kidney disease (PKD) is caused by T. bryosalmonae, which instead belongs to the class Malacosporea. Climate change is providing more suitable conditions for myxozoan parasites lifecycle, posing a high risk to salmonid aquaculture and contributing to the decline of wild trout populations in North America and Europe. Therefore, the aim of this study was to provide the first proteomic profiles of the host in the search for evasion strategies during single and coinfection with M. cerebralis and T. bryosalmonae.
UNASSIGNED: One group of fish was initially infected with M. cerebralis and another group with T. bryosalmonae. After 30 days, half of the fish in each group were co-infected with the other parasite. Using a quantitative proteomic approach, we investigated proteomic changes in the caudal fins and gills of rainbow trout before and after co-infection.
UNASSIGNED: In the caudal fins, 16 proteins were differentially regulated post exposure to M. cerebralis, whereas 27 proteins were differentially modulated in the gills of the infected rainbow trout post exposure to T. bryosalmonae. After co-infection, 4 proteins involved in parasite recognition and the regulation of host immune responses were differentially modulated between the groups in the caudal fin. In the gills, 11 proteins involved in parasite recognition and host immunity, including 4 myxozoan proteins predicted to be virulence factors, were differentially modulated.
UNASSIGNED: The results of this study increase our knowledge on rainbow trout co-infections by myxozoan parasites and rainbow trout immune responses against myxozoans at the portals of entry, supporting a better understanding of these host-parasite interactions.
UNASSIGNED: One group of fish was initially infected with M. cerebralis and another group with T. bryosalmonae. After 30 days, half of the fish in each group were co-infected with the other parasite. Using a quantitative proteomic approach, we investigated proteomic changes in the caudal fins and gills of rainbow trout before and after co-infection.
UNASSIGNED: In the caudal fins, 16 proteins were differentially regulated post exposure to M. cerebralis, whereas 27 proteins were differentially modulated in the gills of the infected rainbow trout post exposure to T. bryosalmonae. After co-infection, 4 proteins involved in parasite recognition and the regulation of host immune responses were differentially modulated between the groups in the caudal fin. In the gills, 11 proteins involved in parasite recognition and host immunity, including 4 myxozoan proteins predicted to be virulence factors, were differentially modulated.
UNASSIGNED: The results of this study increase our knowledge on rainbow trout co-infections by myxozoan parasites and rainbow trout immune responses against myxozoans at the portals of entry, supporting a better understanding of these host-parasite interactions.
摘要：
■对感染粘液虫寄生虫后虹鳟鱼入口的蛋白质组变化知之甚少，大脑粘菌，和苔藓四肽。旋转病（WD）是一种严重的鲑鱼病，由大脑粘质孢子虫引起的，while,增生性肾病（PKD）是由苔藓沙门氏菌引起的，而是属于Malacosporea类。气候变化为粘液虫寄生虫的生命周期提供了更合适的条件，对鲑鱼水产养殖构成高风险，并导致北美和欧洲野生鳟鱼种群减少。因此,这项研究的目的是提供宿主的第一个蛋白质组学图谱，以寻找与大脑M.和苔藓T.salmonae单次感染和共感染期间的逃避策略。
■一组鱼最初感染了大脑分枝杆菌，另一组感染了苔藓沙门氏菌。30天后,每组中有一半的鱼与其他寄生虫共同感染。使用定量蛋白质组学方法，我们研究了共感染前后虹鳟鱼尾鳍和g的蛋白质组学变化。
■在尾鳍中，16种蛋白质在暴露于脑分枝杆菌后差异调节，而暴露于T.bryosalmonae后，感染的虹鳟鱼的g中的27种蛋白质被差异调节。共感染后,参与寄生虫识别和宿主免疫反应调节的4种蛋白质在尾鳍组之间被差异调节。在the中，11种参与寄生虫识别和宿主免疫的蛋白质，包括4种预测为毒力因子的粘虫蛋白，被差分调制。
■这项研究的结果增加了我们对虹鳟鱼由粘液虫寄生虫和虹鳟鱼在入口入口对粘液虫的免疫反应共同感染的知识，支持对这些宿主-寄生虫相互作用的更好理解。
■一组鱼最初感染了大脑分枝杆菌，另一组感染了苔藓沙门氏菌。30天后,每组中有一半的鱼与其他寄生虫共同感染。使用定量蛋白质组学方法，我们研究了共感染前后虹鳟鱼尾鳍和g的蛋白质组学变化。
■在尾鳍中，16种蛋白质在暴露于脑分枝杆菌后差异调节，而暴露于T.bryosalmonae后，感染的虹鳟鱼的g中的27种蛋白质被差异调节。共感染后,参与寄生虫识别和宿主免疫反应调节的4种蛋白质在尾鳍组之间被差异调节。在the中，11种参与寄生虫识别和宿主免疫的蛋白质，包括4种预测为毒力因子的粘虫蛋白，被差分调制。
■这项研究的结果增加了我们对虹鳟鱼由粘液虫寄生虫和虹鳟鱼在入口入口对粘液虫的免疫反应共同感染的知识，支持对这些宿主-寄生虫相互作用的更好理解。
