聚醚醚酮(PEEK)具有优异的生物相容性,抗疲劳性,和骨骼相似的弹性模量,在口腔颌面种植体领域具有广阔的应用前景。然而,PEEK的生物惰性限制了其应用。在这项研究中,我们开发了一种通过简单的连续浸泡过程产生生物相容性和生物活性PEEK的方法,旨在诱导骨分化和增强抗菌性能。最初,通过在浓硫酸和水中浸泡在PEEK表面上引入三维(3D)多孔网络。随后,磺化PEEK表面用氧等离子体处理,然后浸入多巴胺溶液中以涂覆聚多巴胺(PDA)层。最后,通过磷酰氯与表面酚羟基反应获得聚多巴胺磷酸酯改性的3D多孔PEEK。使用扫描电子显微镜进行了系统研究,X射线光电子能谱,水接触角分析,细胞增殖和粘附,成骨基因表达检测,碱性磷酸酶染色,茜素红染色,和细菌培养。总的来说,与未改性的PEEK相比,修饰的PEEK显著增强了体外细胞的增殖和粘附,成骨分化,和抗菌性能。本研究中结合的简单表面修饰措施可能代表了一种有前途的技术,可以促进PEEK在牙科和颌面植入物中的应用。
Polyetheretherketone (PEEK) exhibits excellent biocompatibility, fatigue resistance, and an elastic modulus similar to bone, presenting broad application prospects in the field of dental and maxillofacial implants. However, the bioinertness of PEEK limits its applications. In this study, we developed a method to generate biocompatible and bioactive PEEK through a simple sequential soaking process, aimed at inducing bone differentiation and enhancing antibacterial properties. Initially, a three-dimensional (3D) porous network was introduced on the PEEK surface by soaking in concentrated sulfuric acid and water. Subsequently, the sulfonated PEEK surface was treated with oxygen plasma, followed by immersion in a dopamine solution to coat a polydopamine (PDA) layer. Finally, polydopamine phosphate ester-modified 3D porous PEEK was obtained through the reaction of phosphoryl chloride with surface phenolic hydroxyl groups. Systematic studies were conducted using scanning electron microscopy, X-ray photoelectron spectroscopy, water contact angle analysis, cell proliferation and adhesion, osteogenic gene expression detection, alkaline phosphatase staining, alizarin red staining, and bacterial culture. Overall, compared to unmodified PEEK, the modified PEEK significantly enhanced in vitro cell proliferation and adhesion, osteogenic differentiation, and antibacterial properties. The simple surface modification measures combined in this study may represent a promising technology and could facilitate the application of PEEK in dental and maxillofacial implants.