关键词: 13C NMR FTIR WAXS bombyx mori chitin mulberry silkworm

Mesh : Bombyx / chemistry Animals Chitin / chemistry isolation & purification Spectroscopy, Fourier Transform Infrared X-Ray Diffraction Magnetic Resonance Spectroscopy Morus / chemistry

来  源:   DOI:10.3390/molecules29091914   PDF(Pubmed)

Abstract:
Chitin, a ubiquitous biopolymer, holds paramount scientific and economic significance. Historically, it has been primarily isolated from marine crustaceans. However, the surge in demand for chitin and the burgeoning interest in biopolymers have necessitated the exploration of alternative sources. Among these methods, the mulberry silkworm (Bombyx mori) has emerged as a particularly intriguing prospect. To isolate chitin from Bombyx mori, a chemical extraction methodology was employed. This process involved a series of meticulously orchestrated steps, including Folch extraction, demineralization, deproteinization, and decolorization. The resultant chitin was subjected to comprehensive analysis utilizing techniques such as attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR) spectroscopy, and wide-angle X-ray scattering (WAXS). The obtained results allow us to conclude that the Bombyx mori represents an attractive alternative source of α-chitin.
摘要:
甲壳素,一种无处不在的生物聚合物,具有至关重要的科学和经济意义。历史上,它主要是从海洋甲壳类动物中分离出来的。然而,对甲壳素的需求激增和对生物聚合物的兴趣迅速增长,因此有必要探索替代来源。在这些方法中,桑蚕(Bombyxmori)已经成为一个特别有趣的前景。从家蚕中分离几丁质,采用化学提取方法。这个过程包括一系列精心策划的步骤,包括Folch提取,去矿化,脱蛋白,和脱色。利用衰减全反射-傅里叶变换红外光谱(ATR-FTIR)等技术对所得甲壳质进行综合分析,13C核磁共振(NMR)光谱,和广角X射线散射(WAXS)。获得的结果使我们得出结论,家蚕代表了α-几丁质的有吸引力的替代来源。
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