目的:优化黑桑总黄酮的提取工艺。基于响应面设计,比较黑桑总黄酮含量的差异。新疆不同产地来源及其与抗氧化活性的关系。
方法:使用单向测试来研究乙醇体积分数的影响,料液比,超声功率和超声时间对黑桑总黄酮含量的影响.,Box-Behnken响应面设计优化用于得出最佳提取工艺参数。同时,黑桑的体外抗氧化活性。以羟自由基清除能力、三价铁离子还原能力等抗氧化活性指标进行评价。
结果:最佳提取条件为45%体积乙醇,1∶20g/mL料液比,300W超声功率和60min超声时光。黑桑的总黄酮含量。从六个起源中检测到s,结果表明库车&gt;喀什&gt;库车乌洽镇&gt;和田&gt;英吉沙&gt;玉田,其中库车桑树总黄酮含量为(27.159±0.091)mg/g。黑桑总黄酮含量的差异。不同来源的s差异有统计学意义(P<0.05)。体外抗氧化活性分析表明,库车和和田桑树的抗氧化活性更强,和羟基自由基清除能力,三价铁离子还原能力,不同产地桑树总抗氧化能力差异有统计学意义(P<0.05)。相关性分析表明,黑桑总黄酮含量。与提高羟基自由基清除能力相关。
结论:超声波辅助提取黑桑总黄酮的方法。很简单,并且所构建的模型具有高度的拟合度,能较好地比较黑桑的总黄酮含量。来自新疆的不同产地。
OBJECTIVE: Optimization of the extraction process of total flavonoids from
Morus nigra Linn. based on response surface design, to compare the differences in total flavonoid content of
Morus nigra Linn. from different origins in Xinjiang and its relationship with antioxidant activity.
METHODS: A one-way test was used to investigate the effects of ethanol volume fraction, material-liquid ratio, ultrasonic power and ultrasonic time on total flavonoid content analysis of
Morus nigra Linn. , Box-Behnken response surface design optimisation was used to derive the optimal extraction process parameters. Meanwhile, the in vitro antioxidant activity of
Morus nigra Linn. was evaluated by antioxidant activity indexes such as hydroxyl radical scavenging capacity and ferric ion reducing capacity.
RESULTS: The optimal extraction condition was 45% ethanol by volume, 1∶20 g/mL material-liquid ratio, 300W ultrasound power and 60 min ultrasound time. The total flavonoid content of
Morus nigra Linn. s from six origins was detected and the result showed that Kuche >Kashgar >Kuche Wuqia Town>Hetian >Ying jisha >Yutian, in which the total flavonoid content of mulberry in Kucha city was(27.159±0.091)mg/g. The difference in the total flavonoid content of Morus nigra Linn. s in different origicns was statistically significant(P<0.05). In vitro antioxidant activity analysis showed that the antioxidant activity of mulberry from Kuche and Hetian was stronger, and the hydroxyl radical scavenging capacity, ferric ion reducing capacity, and total antioxidant capacity of mulberry from various origins were statistically significant(P<0.05). The correlation analysis showed that the total flavonoid content of Morus nigra Linn. was correlated with the antioxidant capacity to improve the scavenging of hydroxyl radicals.
CONCLUSIONS: The ultrasonic-assisted method of extracting total flavonoids from Morus nigra Linn. is simple, and the model constructed has a high degree of fit, which can better compare the total flavonoids content of Morus nigra Linn. from different origins in Xinjiang.