PDF(Pubmed)
Abstract：
Nitric oxide (NO) is a gasotransmitter required in a broad range of mechanisms controlling plant development and stress conditions. However, little is known about the specific role of this signaling molecule during lipid storage in the seeds. Here, we show that NO is accumulated in developing embryos and regulates the fatty acid profile through the stabilization of the basic/leucine zipper transcription factor bZIP67. NO and nitro-linolenic acid target and accumulate bZIP67 to induce the downstream expression of FAD3 desaturase, which is misregulated in a non-nitrosylable version of the protein. Moreover, the post-translational modification of bZIP67 is reversible by the trans-denitrosylation activity of peroxiredoxin IIE and defines a feedback mechanism for bZIP67 redox regulation. These findings provide a molecular framework to control the seed fatty acid profile caused by NO, and evidence of the in vivo functionality of nitro-fatty acids during plant developmental signaling.
摘要：
一氧化氮（NO）是控制植物发育和胁迫条件的多种机制所需的气体传递剂。然而,关于这种信号分子在种子脂质储存过程中的具体作用知之甚少。这里,我们表明，NO在发育中的胚胎中积累，并通过稳定碱性/亮氨酸拉链转录因子bZIP67调节脂肪酸谱。NO和硝基亚麻酸靶向并积累bZIP67诱导FAD3去饱和酶的下游表达，在非亚硝基版本的蛋白质中被错误调节。此外，bZIP67的翻译后修饰可通过过氧化物氧化还原蛋白IIE的反硝化活性而逆转,并定义了bZIP67氧化还原调节的反馈机制.这些发现为控制由NO引起的种子脂肪酸谱提供了分子框架，以及硝基脂肪酸在植物发育信号传导过程中体内功能的证据。
