目的:合成生物学是从分子生物学和工程方法中出现的,旨在开发新的,用于从生物计算到药物生产的工业和基础研究应用的生物启发系统。令人惊讶的是,redoxin(硫氧还蛋白,谷氧还蛋白,过氧还蛋白)和其他基于硫醇的氧化还原系统尚未广泛用于许多这些合成生物学应用中。
方法:我们回顾了基于硫醇的氧化还原体系以及使用硫醇依赖性部分的合成生物学应用的发展。
结果:促进细胞质二硫键合的电路的发展,生物计算和肠道疾病的治疗是使用基于硫醇的部分的应用之一。我们建议基因编码的氧化还原传感器,基于硫醇的生物材料和细胞内过氧化氢产生剂也可以是合成生物学应用的有价值的组分。
结论:基于硫醇的系统在细胞氧化还原代谢中起着多种作用,抗氧化剂防御和信号,因此可以提供广泛多样的成分组合,合成生物学应用的零件和设备。然而,限制合成生物学应用采用氧化还原系统的因素包括基于硫醇的组分的正交性,在表征基于硫醇的系统的方法的局限性和对这些系统的设计原理的不完全理解。
OBJECTIVE: Synthetic biology has emerged from molecular biology and engineering approaches and aims to develop novel, biologically-inspired systems for industrial and basic research applications ranging from biocomputing to drug production. Surprisingly,
redoxin (thioredoxin, glutaredoxin, peroxiredoxin) and other thiol-based redox systems have not been widely utilized in many of these synthetic biology applications.
METHODS: We reviewed thiol-based redox systems and the development of synthetic biology applications that have used thiol-dependent parts.
RESULTS: The development of circuits to facilitate cytoplasmic disulfide bonding, biocomputing and the treatment of intestinal bowel disease are amongst the applications that have used thiol-based parts. We propose that genetically encoded redox sensors, thiol-based biomaterials and intracellular hydrogen peroxide generators may also be valuable components for synthetic biology applications.
CONCLUSIONS: Thiol-based systems play multiple roles in cellular redox metabolism, antioxidant defense and signaling and could therefore offer a vast and diverse portfolio of components, parts and devices for synthetic biology applications. However, factors limiting the adoption of
redoxin systems for synthetic biology applications include the orthogonality of thiol-based components, limitations in the methods to characterize thiol-based systems and an incomplete understanding of the design principles of these systems.