硝基脂肪酸(NO2-FAs)是由不饱和脂肪酸与一氧化氮(NO)或NO相关分子相互作用产生的新型分子。在植物中,最近有报道称,NO2-FAs引发抗氧化剂和对压力情况的防御反应。NO2-FAs的特性突出了释放NO的能力,因此通过翻译后修饰(NO-PTM)调节特定的蛋白质靶标。因此,基于NO2-FAs作为生理NO供体的能力,并使用高精度质谱方法,在这里,我们表明内源性硝基亚麻酸(NO2-Ln)可以调节拟南芥中S-亚硝基谷胱甘肽(GSNO)的生物合成。通过LC-MS/MS分析NO2-Ln与GSH的孵育,并记录GSNO的体外合成。这种行为的体内确认是通过在整个根部用15N标记的NO2-Ln孵育拟南芥植物来进行的,在叶片中检测到15N标记的GSNO(GS15NO)。为了深入研究植物中NO2-FA和GSNO的关系,使用调节NO2-FA水平的拟南芥烯醛还原酶突变体(aer突变体)。我们的结果构成了这些新型NO2-FAs调节植物中关键NO生物储库(GSNO)的第一个证据,增加对植物中S-亚硝基硫醇和GSNO信号通路的了解。
Nitro-fatty acids (NO2-FAs) are novel molecules resulting from the interaction of unsaturated fatty acids and nitric oxide (NO) or NO-related molecules. In plants, it has recently been described that NO2-FAs trigger an antioxidant and a defence response against stressful situations. Among the properties of NO2-FAs highlight the ability to release NO therefore modulating specific protein targets through post-translational modifications (NO-PTMs). Thus, based on the capacity of NO2-FAs to act as physiological NO donors and using high-accuracy mass-spectrometric approaches, herein, we show that endogenous nitro-linolenic acid (NO2-Ln) can modulate S-nitrosoglutathione (GSNO) biosynthesis in Arabidopsis. The incubation of NO2-Ln with GSH was analyzed by LC-MS/MS and the in vitro synthesis of GSNO was noted. The in vivo confirmation of this behavior was carried out by incubating Arabidopsis plants with 15N-labeled NO2-Ln throughout the roots, and 15N-labeled GSNO (GS15NO) was detected in the leaves. With the aim to go in depth in the relation of NO2-FA and GSNO in plants, Arabidopsis alkenal reductase mutants (aer mutants) which modulate NO2-FAs levels were used. Our results constitute the first evidence of the modulation of a key NO biological reservoir in plants (GSNO) by these novel NO2-FAs, increasing knowledge about S-nitrosothiols and GSNO-signaling pathways in plants.