PDF(Pubmed)
Abstract：
Zur (zinc uptake regulator) is a significant member of the Fur (ferric uptake regulator) superfamily, which is widely distributed in bacteria. Zur plays crucial roles in zinc homeostasis and influences cell development and environmental adaptation in various species. Yersinia pseudotuberculosis is a Gram-negative enteric that pathogen usually serves as a model organism in pathogenicity studies. The regulatory effects of Zur on the zinc transporter ZnuABC and the protein secretion system T6SS have been documented in Y. pseudotuberculosis. In this study, a comparative transcriptomics analysis between a ∆zur mutant and the wild-type (WT) strain of Y. pseudotuberculosis was conducted using RNA-seq. This analysis revealed global regulation by Zur across multiple functional categories, including membrane transport, cell motility, and molecular and energy metabolism. Additionally, Zur mediates the homeostasis not only of zinc but also ferric and magnesium in vivo. There was a notable decrease in 35 flagellar biosynthesis and assembly-related genes, leading to reduced swimming motility in the ∆zur mutant strain. Furthermore, Zur upregulated multiple simple sugar and oligopeptide transport system genes by directly binding to their promoters. The absence of Zur inhibited biofilm formation as well as reduced resistance to chloramphenicol and acidic stress. This study illustrates the comprehensive regulatory functions of Zur, emphasizing its importance in stress resistance and pathogenicity in Y. pseudotuberculosis.
OBJECTIVE: Bacteria encounter diverse stresses in the environment and possess essential regulators to modulate the expression of genes in responding to the stresses for better fitness and survival. Zur (zinc uptake regulator) plays a vital role in zinc homeostasis. Studies of Zur from multiple species reviewed that it influences cell development, stress resistance, and virulence of bacteria. Y. pseudotuberculosis is an enteric pathogen that serves a model organism in the study of pathogenicity, virulence factors, and mechanism of environmental adaptation. In this study, transcriptomics analysis of Zur\'s regulons was conducted in Y. pseudotuberculosis. The functions of Zur as a global regulator in metal homeostasis, motility, nutrient acquisition, glycan metabolism, and nucleotide metabolism, in turn, increasing the biofilm formation, stress resistance, and virulence were reviewed. The importance of Zur in environmental adaptation and pathogenicity of Y. pseudotuberculosis was emphasized.
OBJECTIVE: Bacteria encounter diverse stresses in the environment and possess essential regulators to modulate the expression of genes in responding to the stresses for better fitness and survival. Zur (zinc uptake regulator) plays a vital role in zinc homeostasis. Studies of Zur from multiple species reviewed that it influences cell development, stress resistance, and virulence of bacteria. Y. pseudotuberculosis is an enteric pathogen that serves a model organism in the study of pathogenicity, virulence factors, and mechanism of environmental adaptation. In this study, transcriptomics analysis of Zur\'s regulons was conducted in Y. pseudotuberculosis. The functions of Zur as a global regulator in metal homeostasis, motility, nutrient acquisition, glycan metabolism, and nucleotide metabolism, in turn, increasing the biofilm formation, stress resistance, and virulence were reviewed. The importance of Zur in environmental adaptation and pathogenicity of Y. pseudotuberculosis was emphasized.
摘要：
Zur（锌吸收调节剂）是Fur（铁吸收调节剂）超家族的重要成员，在细菌中广泛分布。Zur在锌稳态中起着至关重要的作用，并影响各种物种的细胞发育和环境适应。假结核耶尔森氏菌是一种革兰氏阴性肠道病原体，通常在致病性研究中用作模型生物。Zur对锌转运蛋白ZnuABC和蛋白质分泌系统T6SS的调节作用已在假结核中得到证实。在这项研究中,我们使用RNA-seq,对Δzur突变体和假结核Y的野生型(WT)株进行了比较转录组学分析.这一分析揭示了祖尔在多个功能类别中的全球监管，包括膜运输，细胞运动性,分子和能量代谢。此外,Zur不仅在体内介导锌的稳态，而且还介导铁和镁的稳态。35个鞭毛生物合成和组装相关基因显着减少，导致Δzur突变株的游泳运动降低。此外，Zur通过直接结合其启动子来上调多个简单糖和寡肽转运系统基因。缺乏Zur抑制了生物膜的形成以及对氯霉素和酸性胁迫的抗性降低。这项研究说明了祖尔的综合监管功能，强调其在Y.假结核的抗逆性和致病性中的重要性。
目的：细菌在环境中遇到不同的胁迫，并具有必需的调节因子来调节基因的表达，以应对胁迫，从而更好地适应和生存。Zur（锌吸收调节剂）在锌稳态中起着至关重要的作用。来自多个物种的Zur的研究综述了它影响细胞发育，抗应力，和细菌的毒力。Y.假结核是一种肠道病原体，在致病性研究中充当模型生物，毒力因子，和环境适应机制。在这项研究中,在假结核中进行了Zur调节子的转录组学分析。Zur在金属稳态中作为全球调节剂的功能，运动性,营养获取，聚糖代谢，和核苷酸代谢，反过来,增加生物膜的形成，抗应力，并对毒力进行了审查。Zur在环境适应和致病性方面的重要性。强调了假结核。
目的：细菌在环境中遇到不同的胁迫，并具有必需的调节因子来调节基因的表达，以应对胁迫，从而更好地适应和生存。Zur（锌吸收调节剂）在锌稳态中起着至关重要的作用。来自多个物种的Zur的研究综述了它影响细胞发育，抗应力，和细菌的毒力。Y.假结核是一种肠道病原体，在致病性研究中充当模型生物，毒力因子，和环境适应机制。在这项研究中,在假结核中进行了Zur调节子的转录组学分析。Zur在金属稳态中作为全球调节剂的功能，运动性,营养获取，聚糖代谢，和核苷酸代谢，反过来,增加生物膜的形成，抗应力，并对毒力进行了审查。Zur在环境适应和致病性方面的重要性。强调了假结核。
