Abstract：
Two-dimensional gel electrophoresis (2-DE) is a proteomic tool used for the separation of protein mixtures according to protein isoelectric point and molecular mass. Although gel-free quantitative and qualitative proteomic study techniques are now available, 2-DE remains a useful analytical tool. The presented protocol was performed to analyze the flower and leaf proteome of common buckwheat using 24 cm immobilized pH gradient strips (pH 4-7) and visualization of proteins on gels via colloidal Coomassie G-250 staining.
摘要：
二维凝胶电泳（2-DE）是一种蛋白质组学工具，用于根据蛋白质等电点和分子量分离蛋白质混合物。虽然无凝胶定量和定性蛋白质组学研究技术现在可用，2-DE仍然是有用的分析工具。使用24cm固定的pH梯度条（pH4-7）和通过胶体考马斯G-250染色在凝胶上的蛋白质可视化，进行了提出的方案来分析普通荞麦的花和叶蛋白质组。
