{Reference Type}: Journal Article
{Title}: Two-Dimensional Gel Electrophoresis in Studies of Flower and Leaf Proteome of Common Buckwheat.
{Author}: Kopeć P;Krzewska M;Płażek A;
{Journal}: Methods Mol Biol
{Volume}: 2791
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3794-4_12
{Abstract}: Two-dimensional gel electrophoresis (2-DE) is a proteomic tool used for the separation of protein mixtures according to protein isoelectric point and molecular mass. Although gel-free quantitative and qualitative proteomic study techniques are now available, 2-DE remains a useful analytical tool. The presented protocol was performed to analyze the flower and leaf proteome of common buckwheat using 24 cm immobilized pH gradient strips (pH 4-7) and visualization of proteins on gels via colloidal Coomassie G-250 staining.