%0 Journal Article
%T Two-Dimensional Gel Electrophoresis in Studies of Flower and Leaf Proteome of Common Buckwheat.
%A Kopeć P
%A Krzewska M
%A Płażek A
%J Methods Mol Biol
%V 2791
%N 0
%D 2024
%M 38532098
暂无%R 10.1007/978-1-0716-3794-4_12
%X Two-dimensional gel electrophoresis (2-DE) is a proteomic tool used for the separation of protein mixtures according to protein isoelectric point and molecular mass. Although gel-free quantitative and qualitative proteomic study techniques are now available, 2-DE remains a useful analytical tool. The presented protocol was performed to analyze the flower and leaf proteome of common buckwheat using 24 cm immobilized pH gradient strips (pH 4-7) and visualization of proteins on gels via colloidal Coomassie G-250 staining.