Abstract：
The study of the localization of secondary metabolites in both plants and the cell cultures on the intravital sections is hampered by the difficulty of obtaining thin, correctly oriented sections. Techniques for fixing tissues in resins allow these difficulties to be overcome. Properly selected tissue fixation techniques allow using different dyes to identify the compound of interest. In addition, some components of tissue fixation can act as fixatives and as a dye for identifying secondary metabolites. For example, osmium tetroxide, which fixes lipids in tissues, stains phenolic compounds black. This paper describes methods for the detection of phenolic compounds in morphogenic callus culture of buckwheat using osmium tetroxide, Toluidine Blue O dye, and ferric chloride as dyes in epoxy resin-embedded cell culture with double fixation of the material and when material fixed in Karnovsky\'s fixative.
摘要：
在植物和活体切片上的细胞培养物中的次生代谢物定位的研究受到难以获得的阻碍，正确定向的部分。用于将组织固定在树脂中的技术允许克服这些困难。适当选择的组织固定技术允许使用不同的染料来鉴定感兴趣的化合物。此外,组织固定的某些成分可以用作固定剂和鉴定次级代谢产物的染料。例如,四氧化锇,将脂质固定在组织中，将酚类化合物染成黑色。本文介绍了使用四氧化锇检测荞麦形态发生愈伤组织培养物中酚类化合物的方法。甲苯胺蓝O染料，和氯化铁作为染料在环氧树脂包埋的细胞培养中进行双重固定，并将材料固定在Karnovsky的固定剂中。
