Pesticides has transformed the agricultural industry, primarily by enhancing productivity. However, the indiscriminate use of such compounds can adversely affect human health and disrupt ecosystem balance. Limited knowledge exists regarding the removal of these compounds from water, particularly for organophosphate pesticides when employing conventional treatment technologies. Therefore, this study aimed to assess the removal of acephate (ACE) and methamidophos (MET) - considered priority pesticides in Brazil - from waters with high and low turbidity during the clarification process carried out with aluminum sulfate (AS) and ferric chloride (FC), either alone or combined with powdered activated carbon (PAC) adsorption. All water samples were submitted to solid phase extraction (SPE C18 cartridges) prior to acephate and methamidophos analysis by HPLC MS/MS. The clarification process with either AS or FC coagulant did not efficiently remove acephate or methamidophos and maximum average removal (27 %) was observed with waters of high turbidity when using ferric chloride as coagulant. Addition of mineral PAC was also ineffective for removing both pesticides. However, the use of vegetable PAC (10 mg/L) resulted in better removal percentages, up to 80%, but only for methamidophos. The limited removal rates were attributed to the high hydrophilicity of acephate and methamidophos, along with their neutral charge at coagulation pH. These factors hinder the interaction of such organophosphorus pesticides with the flocs formed during coagulation as well as with PAC surface.

Chemical coagulation has gained recognition as an effective technique to enhance the removal efficiency of pollutants in wastewater prior to their entry into a constructed wetland (CW) system. However, its potential impact on the chemical and microbial properties of soil and plant systems within CWs requires further research. This study investigated the impact of using ferric chloride (FeCl3) as a pre-treatment stage for dairy wastewater (DWW) on the chemical and microbial properties of water-soil-plant systems of replicated pilot-scale CWs, comparing them to CWs treating untreated DWW. CWs treating amended DWW had better performance than CWs treating raw DWW for all water quality parameters (COD, TSS, TP, and TN), ensuring compliance with the EU wastewater discharge directives. Soil properties remained mostly unaffected except for pH, calcium and phosphorus (P), which were lower in CWs treating amended DWW. As a result of lower nitrogen (N) and P loads, the plants in CWs receiving FeCl3-amended DWW had lower N and P contents than the plants of raw DWW CWs. However, the lower loads of P into amended DWW CWs did not limit the growth of Phragmites australis, which were able to accumulate trace elements higher than CWs receiving raw DWW. Alpha and Beta-diversity analysis revealed minor differences in community richness and composition between both treatments, with only 3.7% (34 genera) showed significant disparities. Overall, the application of chemical coagulation produced superior effluent quality without affecting the properties of soil and plant of CWs or altering the functioning of the microbial community.

BACKGROUND: An iron overload status induces ferroptosis, an iron-dependent nonapoptotic cell death, in various pathological conditions. We previously reported that hemin (heme), protoporphyrin-IX with ferric iron, activates platelets via C-type lectin-like receptor-2 (CLEC-2) and glycoprotein VI/FcRγ, but protoporphyrin-IX alone blocks CLEC-2-dependent platelet activation. Therefore, we hypothesized that free iron has the ability to activate platelets.
OBJECTIVE: This study aimed to elucidate platelet activation mechanisms of iron (ferric chloride), including the identification of signaling pathways and receptors, and to examine whether platelets regulate ferroptosis.
METHODS: Platelet aggregometry, platelet activation marker expression, and protein phosphorylation were examined in ferric chloride-stimulated human and murine platelets. Inhibitors of platelet activation signaling pathways and receptor-deleted platelets were utilized to identify the responsible signaling pathway and receptor. The effect of platelets on ferroptosis of endothelial cells was investigated in vitro.
RESULTS: Ferric chloride induced platelet activation dependent on Src family kinase pathways in humans and mice. Ferric chloride-induced platelet aggregation was almost lost in CLEC-2-depleted murine platelets and wild-type platelets preincubated with recombinant CLEC-2 proteins. Furthermore, coculture of wild-type platelets, but not CLEC-2-deficient platelets, attenuated ferroptosis of endothelial cells in vitro.
CONCLUSIONS: Ferric chloride activates platelets via CLEC-2 and Src family kinase pathways, and platelets have a protective role in the ferroptosis of endothelial cells dependent on CLEC-2.

With the continuous integration of semiconductor devices, the requirements of the size accuracy and surface quality of etched lead frames are stricter. The etchant is a key factor in the etching process and etched surface quality, while the effects of the difference in etchants on the etched surface morphology of Cu alloy have not been directly studied. In this study, aqua regia, acidic FeCl3 and two CuCl2 solutions were used as etchants, and different CuCrSn specimens were etched and characterized. The results show that the etching rate in aqua regia is high, and the grain orientation, grain boundary (GB) and dislocations have significant influences on the local etching rate. The preferential etching of some atomic planes forms steps between the grains with different orientations, and preferential etching around the GB and dislocation group forms grooves, resulting in high surface roughness. For the surfaces etched by the FeCl3 and CuCl2 etchants, the steps and grooves are blurred; thus, they are less rough. The CuCrSn alloy surface etched by the aqua regia is clean, with little Cr-rich particles, while high-density Cr-rich particles remain on the surfaces etched by the FeCl3 and CuCl2 etchants. For the same kind of etchant, the ion concentration can affect the etching mechanism, rate and the etched surface morphology.

The study of the localization of secondary metabolites in both plants and the cell cultures on the intravital sections is hampered by the difficulty of obtaining thin, correctly oriented sections. Techniques for fixing tissues in resins allow these difficulties to be overcome. Properly selected tissue fixation techniques allow using different dyes to identify the compound of interest. In addition, some components of tissue fixation can act as fixatives and as a dye for identifying secondary metabolites. For example, osmium tetroxide, which fixes lipids in tissues, stains phenolic compounds black. This paper describes methods for the detection of phenolic compounds in morphogenic callus culture of buckwheat using osmium tetroxide, Toluidine Blue O dye, and ferric chloride as dyes in epoxy resin-embedded cell culture with double fixation of the material and when material fixed in Karnovsky\'s fixative.

Anaerobic fermentation (AF) has been identified as a promising method of transforming waste activated sludge (WAS) into high-value products (e.g., short-chain fatty acids (SCFAs)). This study developed thiosulfate/FeCl3 pre-treatment and investigated the effects of different thiosulfate/FeCl3 ratios (S:Fe = 3:1, 3:2, 1:1, 3:4 and 3:5) on SCFA production and sulfur transformation during the AF of WAS. At a S:Fe ratio of 1:1, the maximal SCFA yield (933.3 mg COD/L) and efficient H2S removal (96.5 %) were obtained. S:Fe ratios ≤ 1:1 not only benefited hydrolysis and acidification but largely mitigated H2S generation. These results were supported by the enriched acidogens and reduced sulfur-reducing bacteria (SRB). Molecular ecological network analysis further revealed that the keystone taxon (g_Saccharimonadales) was found in S:Fe = 1:1, together with reductions in associations among methanogens, acidogens and SRB. This work provides a strategy for enhancing high-value product recovery from WAS and minimising H2S emissions.

During coal combustion, the harmful element arsenic can be released into environment and cause potential significant harm to human beings. Therefore, it is very important to study the removal of arsenic from coal before combustion. In this work, simulated SO2-containing flue gas was used to leach arsenic from coal in a 1 L UV photoreactor. The effects of FeCl3, ultraviolet (UV), pH and the Cl-/Fe3+ molar ratio on arsenic leaching and SO2 removal were experimentally investigated and the enhancing mechanism was analysed. Experimental results demonstrated that FeCl3 and UV could efficiently increase iron and arsenic leaching percentages and SO2 removal efficiency. UV irradiation could induce the oxidation of most trivalent arsenic. The arsenic leaching percentage was significantly larger than that of iron. Low pH was favourable for iron and arsenic leaching. The optimal Cl-/Fe3+ molar ratio was determined to be 3:1. The introduced ferric chloride could not only increase the concentrations of free radicals and ferric iron oxidants, the chloride ion might also impede the formation of passive coatings, thus increasing the arsenic leaching percentage, intensifying the oxidation of trivalent arsenic and enhancing the removal of SO2.

In the acidic medium, hydrosulfuryl(-SH) in cysteine hydrochloride can reduce Fe3+ to Fe2+, then Fe2+ react with potassium ferricyanide to form KFe[Fe(CN)6](soluble Prussian blue). Prussian blue has a maximum absorption at 727 nm, Bill\'s law is observed between mass concentration of cysteine hydrochloride and absorbance of Prussian blue, the content of cysteine hydrochloride is indirectly determinated by measuring the absorbance of Prussian blue. An accurate, simple, fast spectrophotometric method for the determination of cysteine hydrochloride content by ferric chloride-potassium ferricyanide has been established. The optimal determination conditions of cysteine hydrochloride content are explored. The cysteine hydrochloride content is determinate by this method.

As an environmentally friendly lignocellulosic biomass separation technology, hydrothermal pretreatment (HP) has a strong application prospect. However, the low separation efficiency is a main factor limiting its application. In this study, the poplar components were separated using HP with ferric chloride and pH buffer (HFB). The optimal conditions were ferric chloride concentration of 0.10 M, reaction temperature of 150 °C, reaction time of 15 min and pH 1.9. The separation of hemicellulose was increased 34.03 % to 77.02 %. The pH buffering resulted in the highest cellulose and lignin retention yields compared to ferric chloride pretreatment (FC). The high efficiency separation of hemicellulose via HFB pretreatment inhibited the degradation of xylose. The hydrolysate was effectively reused for five times. The fiber crystallinity index reached 60.05 %, and the highest C/O ratio was obtained. The results provide theoretical support for improving the efficiency of HP and promoting its application.

Most wastewater treatment facilities that satisfy stricter discharge restrictions for nutrients, remove dissolved inorganic nitrogen (DIN) species efficiently, leaving dissolved organic nitrogen (DON) to be present at a higher proportion (up to 85 %) of total nitrogen (TN) in the effluent. Discharged DON promotes algae growth in receiving water bodies and is a growing concern in effluent potable reuse applications considering its potential to form hazardous nitrogenous disinfection byproducts (N-DBPs). Enhanced coagulation is an established process in the advanced water treatment train for most potable reuse applications. However, so far, no information has been collected at the pilot scale to address DON removal efficiency and process implications by enhanced coagulation under real conditions. This study performed a comprehensive evaluation of DON removal from the effluent of the Truckee Meadows Water Reclamation Facility (TMWRF) by enhanced coagulation over the course of 11 months at the pilot scale. Three different coagulants (aluminum sulfate (alum), poly‑aluminum chloride (PACl), ferric chloride (FC)) and a cationic polymer coagulant aid (Clarifloc) were used. Optimum doses for each coagulant and polymer and ideal pH were determined by jar tests and applied at the pilot. Alum (24 mg/L) resulted in highly variable DON removal (6 % - 40 %, 21 % on average), which was enhanced by the addition of polymer, leading to 32 % DON removal on average. PACl (40 mg/L) and FC (100 mg/L) resulted in more consistent DON removal (on average 45 % and 57 %, respectively); however, polymer addition exerted minimal enhancement for these coagulants. Overall, enhanced coagulation effectively reduced DON in the tertiary effluent at the pilot scale. The treatment showed auxiliary benefits, including dissolved organic carbon (DOC) and orthophosphate removal.