这项研究的目的是评估包括蜂蜜在内的溶液的功效,乙醇,液体石蜡,蒸馏水和柠檬酸(HEFS)作为兔尸体的防腐剂,作为甲醛的潜在替代品。尸体使用三种不同的溶液进行保存:10%福尔马林,35%酒精和HEFS。尸体总共经历了四次采样事件,每隔4个月发生一次,为了收集显微解剖标本,组织学,微生物,真菌学,比色,纹理和气味分析。在硬度方面,解剖和联合移动性指标的适用性,HEFS固定的尸体质量优于福尔马林固定的尸体。HEFS用于组织学分析的固定质量被认为是可接受的,除了肾脏和肠道组织.在纹理分析中,在同一采样周期内,仅弹性参数存在差异(p<0.05)。共鉴定出10株(13.9株)菌,变液杆菌3(30%)主要是金黄色葡萄球菌2(20%),芽孢杆菌,枯草芽孢杆菌,氧化假杆菌,地衣芽孢杆菌,枯草芽孢杆菌亚种。枯草杆菌比例为1(10%),分别,通过微生物和分子分析。然而,无厌氧细菌和真菌分离。相当比例的学生认为HEFS适合在实验室环境中使用,因为它没有令人不快的气味以及对眼睛和呼吸功能的不利影响。总之,我们认为HEFS可以作为福尔马林溶液在兔尸体保存中的可行替代品。
The objective of this study is to assess the efficacy of a solution including honey, ethyl alcohol, liquid paraffin, distilled water and citric acid (HEFS) as a preservative for rabbit cadavers, serving as a potential substitute for formaldehyde. The cadavers underwent preservation using three distinct solutions: 10% formalin, 35% alcohol and HEFS. The cadavers were subjected to a total of four sampling events, occurring at 4-month intervals, in order to collect specimens for microanatomical, histological, microbiological, mycological, colourimetric, texture and odour analysis. In terms of hardness, suitability for dissection and joint mobility metrics, the cadavers fixed with HEFS had superior qualities to those fixed with formalin. The fixation quality of HEFS for histological analyses was deemed acceptable, except kidney and intestinal tissues. In texture analysis, differences only in the elasticity parameter (p < 0.05) in the same sampling period. A total of 10 (13.9) bacteria isolates were identified among which, Metasolibacillus meyeri 3 (30%) was predominantly followed by Staphylococcus aureus 2 (20%), Bacillus siamensis, Bacillus subtilis, Pseudarthrobacter oxydans, Bacillus licheniformis, Bacillus subtilis subsp. subtilis with a proportion of 1 (10%), respectively, by both microbiological and molecular analysis. However, no anaerobic bacteria and fungi were isolated. A considerable percentage of the students had the perception that HEFS was appropriate for utilization in laboratory settings due to its absence of unpleasant odours and detrimental impact on ocular and respiratory functions. In conclusion, we consider that HEFS may serve as a viable substitute for formalin solution in the preservation of rabbit cadavers.