Abstract：
Chronic inflammation at diabetic wound sites results in the uncontrolled accumulation of pro-inflammatory factors and reactive oxygen species (ROS), which impedes cell proliferation and delays wound healing. To promote the healing of diabetic wounds, chitosan/gelatin hydrogels containing ceria nanoparticles (CNPs) of various sizes were created in the current study. CNPs\' efficacy in removing O 2 • - , •OH, and H2O2 was demonstrated, and the scavenging ability of CNPs of varying sizes was compared. The in vitro experiments demonstrated that hydrogels containing CNPs could effectively protect cells from ROS-induced damage and facilitate mouse fibroblast migration. Furthermore, during the treatment of diabetic wounds in vivo, hydrogels containing CNPs exhibited anti-inflammatory activity and could reduce the expression of the pro-inflammatory factors TNF-α (above 30%), IL-6 (above 90%), and IL-1β (above 80%), and effectively promote wound closure (above 80%) by inducing re-epithelialization, collagen deposition, and angiogenesis. In addition, the biological properties and therapeutic effects of hydrogels containing CNPs of various sizes were compared and discussed. The finding revealed that hydrogels with 4 nm CNPs exhibited more significant biological properties and had implications for diabetic wound treatment.
摘要：
糖尿病伤口部位的慢性炎症导致促炎因子和活性氧（ROS）的不受控制的积累，阻碍细胞增殖并延迟伤口愈合。为了促进糖尿病伤口的愈合，壳聚糖/明胶水凝胶含有不同大小的二氧化铈纳米颗粒（CNPs）在目前的研究中创建。CNPs去除O2的功效•-$${\\mathrm{O}}_2^{\\bullet-}$$，•OH,和H2O2被证明，并比较了不同大小的CNPs的清除能力。体外实验表明，含有CNP的水凝胶可以有效保护细胞免受ROS诱导的损伤，并促进小鼠成纤维细胞的迁移。此外，在体内治疗糖尿病伤口期间，含有CNPs的水凝胶具有抗炎活性，可降低促炎因子TNF-α的表达（30%以上），IL-6（90%以上），和IL-1β（80%以上），并通过诱导上皮再生有效促进伤口闭合（80％以上），胶原蛋白沉积，和血管生成。此外,比较和讨论了含有不同大小CNPs的水凝胶的生物学特性和治疗效果。该发现揭示了具有4nmCNP的水凝胶表现出更显著的生物学特性,并且对糖尿病伤口治疗具有意义。
