糖尿病伤口部位的慢性炎症导致促炎因子和活性氧(ROS)的不受控制的积累,阻碍细胞增殖并延迟伤口愈合。为了促进糖尿病伤口的愈合,壳聚糖/明胶水凝胶含有不同大小的二氧化铈纳米颗粒(CNPs)在目前的研究中创建。CNPs去除O2的功效•-$${\\mathrm{O}}_2^{\\bullet-}$$,•OH,和H2O2被证明,并比较了不同大小的CNPs的清除能力。体外实验表明,含有CNP的水凝胶可以有效保护细胞免受ROS诱导的损伤,并促进小鼠成纤维细胞的迁移。此外,在体内治疗糖尿病伤口期间,含有CNPs的水凝胶具有抗炎活性,可降低促炎因子TNF-α的表达(30%以上),IL-6(90%以上),和IL-1β(80%以上),并通过诱导上皮再生有效促进伤口闭合(80%以上),胶原蛋白沉积,和血管生成。此外,比较和讨论了含有不同大小CNPs的水凝胶的生物学特性和治疗效果。该发现揭示了具有4nmCNP的水凝胶表现出更显著的生物学特性,并且对糖尿病伤口治疗具有意义。
Chronic inflammation at diabetic wound sites results in the uncontrolled accumulation of pro-inflammatory factors and reactive oxygen species (ROS), which impedes cell proliferation and delays wound healing. To promote the healing of diabetic wounds, chitosan/gelatin hydrogels containing ceria nanoparticles (CNPs) of various sizes were created in the current study. CNPs\' efficacy in removing O 2 • - , •OH, and H2O2 was demonstrated, and the scavenging ability of CNPs of varying sizes was compared. The in vitro experiments demonstrated that hydrogels containing CNPs could effectively protect cells from ROS-induced damage and facilitate mouse fibroblast migration. Furthermore, during the treatment of diabetic wounds in vivo, hydrogels containing CNPs exhibited anti-inflammatory activity and could reduce the expression of the pro-inflammatory factors TNF-α (above 30%), IL-6 (above 90%), and IL-1β (above 80%), and effectively promote wound closure (above 80%) by inducing re-epithelialization, collagen deposition, and angiogenesis. In addition, the biological properties and therapeutic effects of hydrogels containing CNPs of various sizes were compared and discussed. The finding revealed that hydrogels with 4 nm CNPs exhibited more significant biological properties and had implications for diabetic wound treatment.