背景:Doremaaucheri胶(DAG)是一种苦味的类黄酮胶,广泛用于许多医疗目的,包括伤口恢复。本工作研究了DAG在大鼠切除皮肤损伤中的急性毒性和伤口愈合作用。
方法:将24只SpragueDawley大鼠分为4组,每只大鼠都有全层切除背颈损伤(2.00厘米),并接受0.2毫升以下治疗15天:A组(媒介物),用生理盐水处理的大鼠;B组,大鼠接受内凝胶;C和D,用250和500毫克/千克的DAG处理的大鼠,分别。
结果:结果显示,口服剂量为2和5g/kgDAG的大鼠没有任何毒性症状。与媒介物大鼠相比,在DAG处理后,伤口愈合显著加速,表现为更小的开放区域和更高的伤口收缩百分比。组织学评估显示成纤维细胞形成较高,胶原蛋白沉积,与媒介物大鼠相比,DAG处理的大鼠的颗粒状皮肤组织中炎性细胞浸润明显降低。DAG治疗引起免疫组织化学蛋白(Bax降低和HSP70升高)和炎症介质(TNF-α降低,IL-6和放大的IL-10),与媒介物大鼠相比有显著差异。此外,局部DAG治疗导致羟脯氨酸(HDX)(胶原蛋白)和抗氧化剂含量的显着上调。同时,降低了从DAG处理的大鼠获得的愈合组织中的脂质过氧化(MDA)水平。
结论:DAG目前的伤口收缩可能与其植物化学物质的调节作用有关(多糖,黄酮类化合物,和酚类)在细胞机制上,这证明了它们的民俗使用是合理的,并引发了作为伤口收缩治疗药物添加剂的进一步研究。
BACKGROUND: Dorema aucheri gum (DAG) is a bitter flavonoid gum widely used for numerous medicinal purposes including wound recovery. The present work investigates the acute toxicity and wound-healing effects of DAG in excisional skin injury in rats.
METHODS: Sprague Dawley rats (24) were clustered into four groups, each rat had a full-thickness excisional dorsal neck injury (2.00 cm) and addressed with 0.2 mL of the following treatments for 15 days: Group A (vehicle), rats addressed with normal saline; Group B, rats received intrasite gel; C and D, rats addressed with 250 and 500 mg/kg of DAG, respectively.
RESULTS: The results revealed the absence of any toxic signs in rats who received oral dosages of 2 and 5 g/kg of DAG. Wound healing was significantly accelerated following DAG treatments indicated by smaller open areas and higher wound contraction percentages compared to vehicle rats. Histological evaluation revealed higher fibroblast formation, collagen deposition, and noticeably lower inflammatory cell infiltration in granulated skin tissues of DAG-addressed rats compared to vehicle rats. DAG treatment caused significant modulation of immunohistochemical proteins (decreased Bax and increased HSP 70) and inflammatory mediators (reduced TNF-α, IL-6, and magnified IL-10), which were significantly varied compared to vehicle rats. Moreover, topical DAG treatment led to significant upregulation of the hydroxyproline (HDX) (collagen) and antioxidant content. At the same time, decreased the lipid peroxidation (MDA) levels in healed tissues obtained from DAG-treated rats.
CONCLUSIONS: The present wound contraction by DAG might be linked with the modulatory effect of its phytochemicals (polysaccharides, flavonoids, and phenolic) on the cellular mechanisms, which justify their folkloric use and provokes further investigation as therapeutic drug additives for wound contraction.