PDF(Pubmed)
Abstract：
This research investigated the in-situ decay rates of four human wastewater-associated markers (Bacteroides HF183 (HF183), Lachnospiraceae Lachno3 (Lachno3), cross-assembling phage (crAssphage), pepper mild mottle virus (PMMoV) and three enteric viruses (human adenovirus 40/41 (HAdV 40/41), enterovirus (EV) and human norovirus GII (HNoV GII) in two estuarine water environments (Davidson Park (DP) and Hen and Chicken Bay (HCB) in temperate Sydney, NSW, Australia, employing qPCR and RT-qPCR assays. The study also aimed to compare decay rates observed in mesocosms with previously published laboratory microcosms, providing insights into the persistence of markers and viruses in estuarine environments. Results indicated varying decay rates between DP and HCB mesocosms, with HF183 exhibiting relatively faster decay rates compared to other markers and enteric viruses in sunlight and dark mesocosms. In DP mesocosms, HF183 decayed the fastest, contrasting with PMMoV, which exhibited the slowest. Sunlight induced higher decay rates for all markers and viruses in DP mesocosms. In HCB sunlight mesocosms, HF183 nucleic acid decayed most rapidly compared to other markers and enteric viruses. In dark mesocosms, crAssphage showed the fastest decay, while PMMoV decayed at the slowest rate in both sunlight and dark mesocosms. Comparisons with laboratory microcosms revealed faster decay of markers and enteric viruses in laboratory microcosms than the mesocosms, except for crAssphage and HAdV 40/41 in dark, and PMMoV in sunlight mesocosms. The study concludes that decay rates of markers and enteric viruses vary between estuarine mesocosms, emphasizing the impact of sunlight exposure, which was potentially influenced by the elevated turbidity at HCB estuarine waters. The generated decay rates contribute valuable insights for establishing site-specific risk-based thresholds of human wastewater-associated markers.
摘要：
这项研究调查了四种人类废水相关标记物(拟杆菌HF183(HF183)，蛇床子科Lachno3（Lachno3），交叉组装噬菌体(crAssphage)，辣椒轻度斑驳病毒（PMMoV）和三种肠道病毒（人腺病毒40/41（HAdV40/41），悉尼温带河口水环境中的肠道病毒（EV）和人类诺如病毒GII（HNoVGII），新南威尔士州,澳大利亚,采用qPCR和RT-qPCR测定。该研究还旨在将在中观宇宙中观察到的衰变速率与先前发表的实验室微观世界进行比较，提供对标记和病毒在不同环境中的持久性的见解。结果表明，戴维森公园（DP）和母鸡湾（HCB）介观之间的衰变速率不同，HF183始终表现出更快的衰变，和crAssphage在阳光和黑暗条件下显示不同的衰变率。在DP中观宇宙中，HF183衰减最快，与PMMoV相比，表现出最慢的衰变。阳光诱导所有标记和病毒的衰变率较高。同样，HCB中观表现出不同的衰变率，HF183在阳光下衰减最快。阳光照射显着加速了两个中观的衰变，影响DP介观中的HF183、Lachno3和EV。HF183始终比其他目标衰减得更快，肠道病毒比PMMoV和crAssphage衰减更快。与实验室微观世界的比较揭示了比中观世界更快的衰变，除了在阳光和电动汽车中燃烧。研究得出的结论是，中观之间的衰变速率不同，强调阳光照射的影响，这可能受到六氯代苯浊度升高的影响。HF183始终表现出更快的衰变，在目标之间观察到不同的模式。产生的衰变速率为建立人类废水相关标记基因的基于特定地点风险的阈值提供了有价值的见解。
