PDF(Pubmed)
Abstract：
BACKGROUND: Indotyphlops braminus, the only known triploid parthenogenetic snake, is a compelling species for revealing the mechanism of polyploid emergence in vertebrates.
METHODS: In this study, we applied PacBio isoform sequencing technology to generate the first full-length transcriptome of I. braminus, aiming to improve the understanding of the molecular characteristics of this species.
RESULTS: A total of 51,849 nonredundant full-length transcript assemblies (with an N50 length of 2980 bp) from I. braminus were generated and fully annotated using various gene function databases. Our analysis provides preliminary evidence supporting a recent genome duplication event in I. braminus. Phylogenetic analysis indicated that the divergence of I. braminus subgenomes occurred approximately 11.5 ~ 15 million years ago (Mya). The full-length transcript resource generated as part of this research will facilitate transcriptome analysis and genomic evolution studies in the future.
METHODS: In this study, we applied PacBio isoform sequencing technology to generate the first full-length transcriptome of I. braminus, aiming to improve the understanding of the molecular characteristics of this species.
RESULTS: A total of 51,849 nonredundant full-length transcript assemblies (with an N50 length of 2980 bp) from I. braminus were generated and fully annotated using various gene function databases. Our analysis provides preliminary evidence supporting a recent genome duplication event in I. braminus. Phylogenetic analysis indicated that the divergence of I. braminus subgenomes occurred approximately 11.5 ~ 15 million years ago (Mya). The full-length transcript resource generated as part of this research will facilitate transcriptome analysis and genomic evolution studies in the future.
摘要：
背景：马尾虫，唯一已知的三倍体孤雌生殖蛇，是揭示脊椎动物多倍体出现机制的令人信服的物种。
方法：在本研究中，我们应用PacBio同工型测序技术产生了第一个全长的Braminus转录组，旨在提高对该物种分子特征的理解。
结果：产生了来自I.braminus的总共51,849个非冗余全长转录物组件（N50长度为2980bp），并使用各种基因功能数据库进行了完整注释。我们的分析提供了初步证据，支持最近在I.braminus中发生的基因组重复事件。系统发育分析表明，马蹄铁亚基因组的分歧发生在约11.5~15百万年前(Mya)。作为这项研究的一部分而产生的全长转录本资源将促进将来的转录组分析和基因组进化研究。
方法：在本研究中，我们应用PacBio同工型测序技术产生了第一个全长的Braminus转录组，旨在提高对该物种分子特征的理解。
结果：产生了来自I.braminus的总共51,849个非冗余全长转录物组件（N50长度为2980bp），并使用各种基因功能数据库进行了完整注释。我们的分析提供了初步证据，支持最近在I.braminus中发生的基因组重复事件。系统发育分析表明，马蹄铁亚基因组的分歧发生在约11.5~15百万年前(Mya)。作为这项研究的一部分而产生的全长转录本资源将促进将来的转录组分析和基因组进化研究。
