{Reference Type}: Journal Article
{Title}: Polyploidization of Indotyphlops braminus: evidence from isoform-sequencing.
{Author}: Zhu F;Lu J;Sun K;Deng C;Xu Y;
{Journal}: BMC Genom Data
{Volume}: 25
{Issue}: 1
{Year}: 2024 Feb 26
暂无{DOI}: 10.1186/s12863-024-01208-y
{Abstract}: <B>BACKGROUND: </B>Indotyphlops braminus, the only known triploid parthenogenetic snake, is a compelling species for revealing the mechanism of polyploid emergence in vertebrates.<BR><B>METHODS: </B>In this study, we applied PacBio isoform sequencing technology to generate the first full-length transcriptome of I. braminus, aiming to improve the understanding of the molecular characteristics of this species.<BR><B>RESULTS: </B>A total of 51,849 nonredundant full-length transcript assemblies (with an N50 length of 2980 bp) from I. braminus were generated and fully annotated using various gene function databases. Our analysis provides preliminary evidence supporting a recent genome duplication event in I. braminus. Phylogenetic analysis indicated that the divergence of I. braminus subgenomes occurred approximately 11.5 ~ 15 million years ago (Mya). The full-length transcript resource generated as part of this research will facilitate transcriptome analysis and genomic evolution studies in the future.