Both the absolute (~ × 26) and relative (~ × 7) number of HCM-related publications increased from 1974 to 2020. There was a clear bias towards males and individually housed animals, but during the past decade (2011-2020), an increasing number of studies used both sexes and group housing. In most studies, animals were kept for short (up to 4 weeks) time periods in the HCM systems; intermediate time periods (4-12 weeks) increased in frequency in the years between 2011 and 2020. Before the 2000s, HCM techniques were predominantly applied for less than 12 h, while 24-h measurements have been more frequent since the 2000s. The systematic review demonstrated that manual monitoring is decreasing in relation to automatic techniques but still relevant. Until (and including) the 1990s, most techniques were applied manually but have been progressively replaced by automation since the 2000s. Independent of the year of publication, the main behavioral parameters measured were locomotor activity, feeding, and social behaviors; the main physiological parameters were heart rate and electrocardiography. External appearance-related parameters were rarely examined in the home cages. Due to technological progress and application of artificial intelligence, more refined and detailed behavioral parameters have been investigated in the home cage more recently.
Over the period covered in this study, techniques for HCM of mice and rats have improved considerably. This development is ongoing and further progress as well as validation of HCM systems will extend the applications to allow for continuous, longitudinal, non-invasive monitoring of an increasing range of parameters in group-housed small rodents in their home cages.
结果:从1974年到2020年,与HCM相关的出版物的绝对(〜×26)和相对(〜×7)数量均增加。对雄性和单独饲养的动物有明显的偏见,但在过去十年(2011-2020年),越来越多的研究同时使用性别和群体住房。在大多数研究中,动物在HCM系统中饲养时间较短(长达4周);在2011年至2020年期间,中间时间段(4-12周)的频率增加.在2000年之前,HCM技术主要应用不到12小时,而24小时测量自2000年代以来更加频繁。系统审查表明,与自动技术相关的手动监控正在减少,但仍然相关。直到(包括)1990年代,大多数技术都是手动应用的,但自2000年代以来已逐渐被自动化所取代。独立于出版之年,测量的主要行为参数是运动活动,喂养,和社会行为;主要生理参数为心率和心电图。很少在家庭笼子中检查与外观相关的参数。由于人工智能的技术进步和应用,更精细和详细的行为参数已经在家庭笼子最近进行了调查。
结论:在本研究期间,小鼠和大鼠的HCM技术有了很大改善。此开发正在进行中,进一步的进展以及HCM系统的验证将扩展应用程序,以允许连续,纵向,非侵入性监测家庭笼中群居的小型啮齿动物参数范围的增加。