Abstract：
Signal-mediated cell fusion is vital for colony development in filamentous fungi. Arthrobotrys oligospora is a representative nematode-trapping (NT) fungus that produces adhesive networks (traps) to capture nematodes. Here, we characterized Aoadv-1, Aoso, Aoham-6, and Aoham-5 of A. oligospora, homologs of proteins involved in cellular communication and fusion in the model fungus Neurospora crassa. The deletion of four genes resulted in the complete loss of cell fusion, and traps produced by mutants did not close to form mycelial rings but were still capable of capturing nematodes. The absence of these genes inhibits aerial mycelial extension, slows colony growth, and increases mycelial branching. In addition, the mutants showed reduced sporulation capacity and tolerance to oxidative stress, increased sensitivity to SDS, and disturbed lipid droplet accumulation and autophagy. In addition, transcriptome and metabolomic analyses suggested that Aoadv-1 and Aoso are involved in multiple cellular processes and secondary metabolism. Our results revealed that Aoadv-1, Aoso, Aoham-6, and Aoham-5 regulate mycelial growth and trap morphogenesis through cell fusion, which contributed to elucidating the molecular mechanisms of cellular communication regulating mycelial development and trap morphogenesis in NT fungi.
摘要：
信号介导的细胞融合对于丝状真菌的菌落发育至关重要。Arthrobotrys寡孢子是一种代表性的线虫捕获（NT）真菌，可产生粘附网络（陷阱）来捕获线虫。这里,我们描述了Aoadv-1，Aoso，A.寡孢子虫的Aoham-6和Aoham-5，在模型真菌Neurosporacrassa中参与细胞通讯和融合的蛋白质的同源物。四个基因的缺失导致细胞融合的完全丧失，突变体产生的陷阱没有形成菌丝体环，但仍然能够捕获线虫。这些基因的缺失抑制了气生菌丝体的延伸，减缓菌落生长，并增加菌丝分枝。此外,突变体显示出产孢能力和对氧化应激的耐受性降低，增加对SDS的敏感性，和扰乱脂滴积累和自噬。此外,转录组和代谢组分析表明，Aoadv-1和Aoso参与多个细胞过程和次级代谢。我们的结果显示，Aoadv-1，Aoso，Aoham-6和Aoham-5通过细胞融合调节菌丝体生长并捕获形态发生，这有助于阐明NT真菌中细胞通讯调节菌丝体发育和陷阱形态发生的分子机制。
