植物病原真菌镰刀菌的菌丝体表现出不同的营养生长结构,无性孢子形成,性发育,毒力,和衣原体孢子的形成.这些结构对真菌的存活和致病性至关重要,需要基于环境线索的精确监管。最初在稻瘟病菌中发现,转录因子Con7p调节分生孢子和感染相关的形态发生,但不是营养生长。我们表征了Con7p直系同源物FgCon7,并且FgCON7的缺失导致分生孢子生产中的严重缺陷,毒力,性发育,和营养生长。缺失突变体的菌丝体转化为具有高甲壳素水平积累的衣原体孢子样结构。值得注意的是,增强FgABAA表达部分缓解了FgCON7缺失突变体中的发育问题。染色质免疫沉淀(ChIP)-定量PCR(qPCR)分析证实了FgABAA和FgCON7之间的直接遗传联系。此外,几丁质合成酶基因Fg6550(FGSG_06550)在FgCON7缺失突变体中显示出显着的上调,和改变FgCON7表达影响细胞壁完整性。进一步的研究将集中在了解几丁质合酶基因的行为及其FgCon7的调控。这项研究为我们理解这种植物病原真菌中调节菌丝分化和分生孢子的遗传途径做出了重要贡献。
目的:子囊菌是小麦和大麦白叶枯病病的主要原因,以及玉米的穗和茎腐烂。鉴于分生孢子和子囊孢子在禾谷镰刀菌疾病周期中的重要性,精确的时空调控这些生物过程是至关重要的。在这项研究中,我们对稻瘟病菌Con7p直系同源物进行了表征,并发现FgCon7显着影响真菌发育和致病性的各个关键方面。值得注意的是,FgABAA的过表达部分恢复了FgCON7缺失突变体的发育缺陷。ChIP-qPCR分析证实了FgABAA和FgCON7之间的直接遗传联系。此外,我们的研究揭示了FgCon7和几丁质积累与几丁质合酶基因表达之间的明显相关性。这些发现为这种植物病原真菌中调节分生孢子的遗传机制和菌丝体分化的重要性提供了有价值的见解。
The mycelium of the plant pathogenic fungus Fusarium graminearum exhibits distinct structures for vegetative growth, asexual sporulation, sexual development, virulence, and chlamydospore formation. These structures are vital for the survival and pathogenicity of the fungus, necessitating precise regulation based on environmental cues. Initially identified in Magnaporthe oryzae, the transcription factor Con7p regulates
conidiation and infection-related morphogenesis, but not vegetative growth. We characterized the Con7p ortholog FgCon7, and deletion of FgCON7 resulted in severe defects in conidium production, virulence, sexual development, and vegetative growth. The mycelia of the deletion mutant transformed into chlamydospore-like structures with high chitin level accumulation. Notably, boosting FgABAA expression partially alleviated developmental issues in the FgCON7 deletion mutant. Chromatin immunoprecipitation (ChIP)-quantitative PCR (qPCR) analysis confirmed a direct genetic link between FgABAA and FgCON7. Furthermore, the chitin synthase gene Fg6550 (FGSG_06550) showed significant upregulation in the FgCON7 deletion mutant, and altering FgCON7 expression affected cell wall integrity. Further research will focus on understanding the behavior of the chitin synthase gene and its regulation by FgCon7 in F. graminearum. This study contributes significantly to our understanding of the genetic pathways that regulate hyphal differentiation and
conidiation in this plant pathogenic fungus.
OBJECTIVE: The ascomycete fungus Fusarium graminearum is the primary cause of head blight disease in wheat and barley, as well as ear and stalk rot in maize. Given the importance of conidia and ascospores in the disease cycle of F. graminearum, precise spatiotemporal regulation of these biological processes is crucial. In this study, we characterized the Magnaporthe oryzae Con7p ortholog and discovered that FgCon7 significantly influences various crucial aspects of fungal development and pathogenicity. Notably, overexpression of FgABAA partially restored developmental defects in the FgCON7 deletion mutant. ChIP-qPCR analysis confirmed a direct genetic link between FgABAA and FgCON7. Furthermore, our research revealed a clear correlation between FgCon7 and chitin accumulation and the expression of chitin synthase genes. These findings offer valuable insights into the genetic mechanisms regulating
conidiation and the significance of mycelial differentiation in this plant pathogenic fungus.