PDF(Pubmed)
Abstract：
Midges are widely distributed globally and can transmit various human and animal diseases through blood-sucking. As part of this study, 259,300 midges were collected from four districts in Yunnan province, China, to detect the viral richness and diversity using metavirome analysis techniques. As many as 26 virus families were detected, and the partial sequences of bluetongue virus (BTV), dengue virus (DENV), and Getah virus (GETV) were identified by phylogenetic analysis and PCR amplification. Two BTV gene fragments, 866 bps for the VP2 gene of BTV type 16 and 655 bps for the VP5 gene of BTV type 21, were amplified. The nucleotide sequence identities of the two amplified BTV fragments were 94.46% and 98.81%, respectively, with two classical BTV-16 (GenBank: JN671907) and BTV-21 strains (GenBank: MK250961) isolated in Yunnan province. Furthermore, the BTV-16 DH2021 strain was successfully isolated in C6/36 cells, and the peak value of the copy number reached 3.13 × 107 copies/μL after five consecutive BHK-21 cell passages. Moreover, two 2054 bps fragments including the E gene of DENV genotype Asia II were amplified and shared the highest identity with the DENV strain isolated in New Guinea in 1944. A length of 656 bps GETV gene sequence encoded the partial capsid protein, and it shared the highest identity of 99.68% with the GETV isolated from Shandong province, China, in 2017. Overall, this study emphasizes the importance of implementing prevention and control strategies for viral diseases transmitted by midges in China.
摘要：
Midges在全球分布广泛，可以通过吸血传播各种人类和动物疾病。作为这项研究的一部分,从云南省四个地区收集了259,300只mid,中国,使用metavirome分析技术检测病毒的丰富度和多样性。检测到多达26个病毒家族，和蓝舌病毒（BTV）的部分序列，登革热病毒（DENV），通过系统发育分析和PCR扩增鉴定了Getah病毒（GETV）。两个BTV基因片段，BTV类型16的VP2基因的866bps和BTV类型21的VP5基因的655bps被扩增。两个扩增的BTV片段的核苷酸序列同一性分别为94.46%和98.81%,分别,在云南省分离出两个经典的BTV-16（GenBank：JN671907）和BTV-21菌株（GenBank：MK250961）。此外，BTV-16DH2021菌株在C6/36细胞中成功分离，连续五次传代BHK-21细胞后，拷贝数的峰值达到3.13×107个拷贝/μL。此外，扩增了两个2054bps片段，包括DENV基因型亚洲II的E基因，并与1944年在巴布亚新几内亚分离的DENV菌株具有最高的同一性。一段长度为656bps的GETV基因序列编码部分衣壳蛋白，它与来自山东省的GETV共享99.68％的最高身份，中国,在2017年。总的来说,这项研究强调了在中国实施预防和控制策略的重要性。
