内罗毕羊病(NSD)是一种典型的蜱传综合征,以严重的出血性胃肠炎为特征,自然流产,小反刍动物的病死率很高。病原体,内罗毕羊病病毒(NSDV),也与人类感染有关,表明其可能的人畜共患潜力。在这项研究之前,从吉林收集的蜱中检测到NSDV,湖北,和中国的辽宁省。在本研究中,在山东省共采集了343只蜱(长钩病),到2020年,中国将合并为16个图书馆。元转录组测序数据的分析从三个池中鉴定了NSDV菌株SDWL07、SDWL08和SDWL16。从未饲喂的蜱中检测到SDWL07和SDWL16菌株,而SDWL08是从牛饲养的蜱中检测到的。系统发育分析表明,与印度和肯尼亚相比,这三个菌株与其他中国NSDV菌株之间的序列同一性更高。系统发育分析还显示,它们聚集在一起,属于中国血统,表明它们之间没有潜在的基因重组。总之,这是山东省首份NSDV鉴定报告,突出了这种病原体不断扩大的流行区域。中国应加强对NSDV的监控,尤其是在长H.longicornis流行的地区.
Nairobi Sheep Disease (NSD) is a typical tick-borne syndrome characterized by severe hemorrhagic gastroenteritis, spontaneous abortion, and a high case fatality rate in small ruminants. The pathogenic agent, Nairobi sheep disease virus (NSDV), has also been associated with human infections, indicating its possible zoonotic potential. Prior to this study, NSDV has been detected from ticks collected in Jilin, Hubei, and Liaoning provinces in China. In the present study, a total of 343 ticks (Haemaphysalis longicornis) were collected in Shandong province, China in 2020, and pooled into 16 libraries. Analysis of the meta-transcriptomic sequencing data identified NSDV strains SDWL07, SDWL08, and SDWL16 from three pools. The SDWL07 and SDWL16 strains were detected from unfed ticks, while SDWL08 was detected from cattle-feeding ticks. Phylogenetic analyses showed higher sequence identities between the three strains and other Chinese NSDV strains than those from India and Kenya. Phylogenetic analyses also revealed that they clustered together and fell within the China lineage, suggesting no potential genetic reassortment among them. In summary, this is the first report of the identification of NSDV in Shandong province, highlighting the continually expanding endemic regions of this pathogen. Surveillance of NSDV should be intensified in China, especially in areas where H. longicornis is endemic.