病毒通过引起影响产量和种子质量的疾病而对大豆生产产生负面影响。新出现或重新出现的病毒也可能威胁大豆生产,因为目前的控制措施可能对它们无效。此外,当没有基于序列或抗体的资源时,新植物病毒的检测和表征需要大量的努力。
在这项研究中,在2016-2019年的生长季节,对大豆田进行了病毒样疾病症状的侦察。从有症状的大豆部位提取总RNA,cDNA文库的制备,使用高通量测序(HTS)进行RNA测序。使用定制的生物信息学工作流程来鉴定和组装已知和未知的病毒基因组。
在单一或混合感染中鉴定了几种病毒。为烟草条纹病毒(TSV)生成了全长或几乎全长的基因组,苜蓿花叶病毒(AMV),烟草环斑病毒(TRSV),大豆矮缩病毒(SbDV),豆荚斑驳病毒(BPMV),大豆静脉坏死病毒(SVNV),三叶草黄静脉病毒(ClYVV),和一种新的病毒命名为大豆病毒1(SIlV1)。回收了两种不同的ClYVV分离株,并在烟草中研究了它们的生物学特性,蚕豆,和大豆。除了个别病毒的感染,我们还发现,各种组合的混合病毒感染相当普遍。
合照,这项研究的结果表明,基于HTS的技术是鉴定田间大豆中几种病毒的有价值的诊断工具,可以提供有关预期病毒以及以前未在大豆中检测到的病毒的快速信息.
Viruses negatively impact soybean production by causing diseases that affect yield and seed quality. Newly emerging or re-emerging viruses can also threaten soybean production because current control measures may not be effective against them. Furthermore, detection and characterization of new plant viruses requires major efforts when no sequence or antibody-based resources are available.
In this study, soybean fields were scouted for virus-like disease symptoms during the 2016-2019 growing seasons. Total RNA was extracted from symptomatic soybean parts, cDNA libraries were prepared, and RNA sequencing was performed using high-throughput sequencing (HTS). A custom bioinformatic workflow was used to identify and assemble known and unknown virus genomes.
Several viruses were identified in single or mixed infections. Full- or nearly full-length genomes were generated for tobacco streak virus (TSV), alfalfa mosaic virus (AMV), tobacco ringspot virus (TRSV), soybean dwarf virus (SbDV), bean pod mottle virus (BPMV), soybean vein necrosis virus (SVNV), clover yellow vein virus (ClYVV), and a novel virus named soybean ilarvirus 1 (SIlV1). Two distinct ClYVV isolates were recovered, and their biological properties were investigated in Nicotiana benthamiana, broad bean, and soybean. In addition to infections by individual viruses, we also found that mixed viral infections in various combinations were quite common.
Taken together, the results of this study showed that HTS-based technology is a valuable diagnostic tool for the identification of several viruses in field-grown soybean and can provide rapid information about expected viruses as well as viruses that were previously not detected in soybean.