PDF(Pubmed)
Abstract：
Zinc (Zn) deficiency is a common limiting factor in agricultural soils, which leads to significant reduction in both the yield and nutritional quality of agricultural produce. Exploring the quantitative trait loci (QTL) for shoot and grain Zn accumulation would help to develop new barley cultivars with greater Zn accumulation efficiency. In this study, two glasshouse experiments were conducted by growing plants under adequate and low Zn supply. From the preliminary screening of ten barley cultivars, Sahara (0.05 mg/pot) and Yerong (0.06 mg/pot) showed the lowest change in shoot Zn accumulation, while Franklin (0.16 mg/pot) had the highest change due to changes in Zn supply for plant growth. Therefore, the double haploid (DH) population derived from Yerong × Franklin was selected to identify QTL for shoot mineral accumulation and biomass production. A major QTL hotspot was detected on chromosome 2H between 31.91 and 73.12 cM encoding genes for regulating shoot mineral accumulations of Zn, Fe, Ca, K and P, and the biomass. Further investigation demonstrated 16 potential candidate genes for mineral accumulation, in addition to a single candidate gene for shoot biomass in the identified QTL region. This study provides a useful resource for enhancing nutritional quality and yield potential in future barley breeding programs.
摘要：
锌（Zn）缺乏是农业土壤中常见的限制因素，这导致农产品的产量和营养质量显著下降。探索芽和籽粒Zn积累的数量性状位点（QTL）将有助于开发具有更高Zn积累效率的大麦新品种。在这项研究中,通过在充足和低锌供应下种植植物进行了两次温室实验。通过对十个大麦品种的初步筛选，撒哈拉沙漠(0.05mg/盆)和叶荣(0.06mg/盆)表现出最低的芽锌积累变化,而富兰克林（0.16mg/盆）由于植物生长的锌供应变化而变化最大。因此,选择来自Yerong×Franklin的双单倍体（DH）种群来鉴定用于茎矿物积累和生物量生产的QTL。在31.91和73.12cM编码基因之间的2H染色体上检测到一个主要的QTL热点，用于调节Zn的芽矿物质积累，Fe,Ca,K和P,和生物量。进一步的调查显示了16个潜在的矿物积累候选基因，除了确定的QTL区域中的芽生物量的单个候选基因之外。这项研究为未来大麦育种计划中提高营养品质和产量潜力提供了有用的资源。
