尽管最近有许多关于非酒精性脂肪性肝病(NAFLD)治疗的研究,最佳治疗方法尚未确定。在这个未完成的项目中,我们将来自肠道微生物群(GM)和大麦(HV)的次级代谢产物(SMs)通过网络药理学(NP)研究其组合效应.此外,我们在组合视角(HV,和GM)。通过蛋白质-蛋白质相互作用(PPI)网络分析了总共31个关键靶标,JUN被确定为NAFLD的最高目标。在泡沫图上,我们揭示了apelin信号通路,具有最低的富集因子拮抗NAFLD。整体来说,我们仔细检查了GBSTM,以确定关键组件(GM,信号通路,目标,和代谢物)与Apelin信号通路相关。因此,我们发现主要的GMs(Eubacteriumlimosum,Eggerthellasp.SDG-2,传入YIT12060的Alistipes,OdoribacterlaneusYIT12061,ParaprevotellaclaraYIT11840,ParaprevotellaxylaniphilaYIT11841)以改善NAFLD。分子对接试验(MDT)提示色胺酮-JUN是一种激动剂,相反,二氢缩水素-HDAC5,1,3-二苯基丙-2-醇-NOS1和(10[(乙酰氧基)甲基]-9-蒽基)乙酸甲酯-NOS2,它们是apelin信号传导途径中的拮抗构象。总的来说,这些结果表明,联合治疗可能是治疗NAFLD的有效策略.
Despite many recent studies on non-alcoholic fatty liver disease (NAFLD) therapeutics, the optimal treatment has yet to be determined. In this unfinished project, we combined secondary metabolites (SMs) from the gut microbiota (GM) and Hordeum vulgare (HV) to investigate their combinatorial effects via network pharmacology (NP). Additionally, we analyzed GM or barley - signalling pathways - targets - metabolites (GBSTMs) in combinatorial perspectives (HV, and GM). A total of 31 key targets were analysed via a protein-protein interaction (PPI) network, and JUN was identified as the uppermost target in NAFLD. On a bubble plot, we revealed that apelin signalling pathway, which had the lowest enrichment factor antagonize NAFLD. Holistically, we scrutinized GBSTM to identify key components (GM, signalling pathways, targets, and metabolites) associated with the Apelin signalling pathway. Consequently, we found that the primary GMs (Eubacterium limosum, Eggerthella sp. SDG-2, Alistipes indistinctus YIT 12060, Odoribacter laneus YIT 12061, Paraprevotella clara YIT 11840, Paraprevotella xylaniphila YIT 11841) to ameliorate NAFLD. The molecular docking test (MDT) suggested that tryptanthrin-JUN is an agonist, conversely, dihydroglycitein-HDAC5, 1,3-diphenylpropan-2-ol-NOS1, and (10[(Acetyloxy)methyl]-9-anthryl)methyl acetate-NOS2, which are antagonistic conformers in the apelin signalling pathway. Overall, these results suggest that combination therapy could be an effective strategy for treating NAFLD.