由柑橘链格孢菌引起的柑橘褐斑是柑橘作物成功生产的新兴威胁之一。本研究,以50个叶片样本的大量样本量进行统计可靠性,旨在确定褐斑病发作后柑橘叶片中矿物质含量的变化。来自各种易感柑橘品种的叶片样品(Valentia晚期,华盛顿肚脐,和Kinnow)和抗性品种(Citron,Eruka柠檬,和Mayer柠檬)进行了分析。在不同反应组(接种和未接种)中观察到矿物质含量的显着变化(p≤0.05),类型(抗性和易感性),和柑橘品种对柑橘链格孢菌感染的反应。方差分析显示柑橘叶片的矿物质水平发生了显着变化,包括氮(N),磷(P),钾(K),钙(Ca),镁(Mg),锌(Zn),钠(Na),铁(Fe),和铜(Cu)。结果表明,N和P的浓度分别为6.63%和1.44%,分别,在抗性植物中,而易感植物表现出6.07%和1.19%的差异。此外,抗性植物表现出更高的钾浓度,Ca,Mg,Zn,Na,Fe,和Cu分别为8.40、2.1、1.83、2.21、1.58、2.89和0.36ppm,与分别显示浓度为5.99、1.93、1.47、1.09、1.24、1.81和0.31ppm的易感植物相比。接种后,柑橘的抗性和易感植物的矿物质含量均降低。N(8.56),P(1.87)%,K(10.74),Ca(2.71),Mg(2.62),Zn(2.20),Na(2.08),在未接种组的柑橘植物中记录到Fe(3.57)和Cu(0.20)ppm,分别降低到3.15和0.76%和3.66、1.40、0.63、0.42、0.74、1.13和0.13ppm。已完成易感品种的离子含量低于抗性品种。抗性柑橘品种中较高浓度的离子含量建立了柑橘植物的生化和生理过程,这有助于限制病原体的传播。进一步的研究可以探索柑橘矿物质营养与抗病性之间的相互作用,有可能导致新的抗病品种的开发。
Brown spot of citrus caused by Alternaria citri is one of the emerging threats to the successful production of citrus crops. The present study, conducted with a substantial sample size of 50 leaf samples for statistical reliability, aimed to determine the change in mineral content in citrus leaves after brown spot disease attack. Leaf samples from a diverse range of susceptible citrus varieties (Valentia late, Washington navel, and Kinnow) and resistant varieties (Citron, Eruka lemon, and Mayer lemon) were analyzed. Significant variations (p ≤ 0.05) in mineral contents were observed across reaction groups (inoculated and un-inoculated), types (resistant and susceptible), and varieties of citrus in response to infection of Alternaria citri. The analysis of variance showed significant changes in mineral levels of citrus leaves, including nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), zinc (Zn), sodium (Na), iron (Fe), and copper (Cu). The results indicate that the concentration of N and P differed by 6.63% and 1.44%, respectively, in resistant plants, while susceptible plants showed a difference of 6.07% and 1.19%. Moreover, resistant plants showed a higher concentrations of K, Ca, Mg, Zn, Na, Fe, and Cu at 8.40, 2.1, 1.83, 2.21, 1.58, 2.89, and 0.36 ppm respectively, compared to susceptible plants which showed concentrations of 5.99, 1.93, 1.47, 1.09, 1.24, 1.81, and 0.31 ppm respectively. Amounts of mineral contents were reduced in both resistant as well as susceptible plants of citrus after inoculation. Amount of N (8.56), P (1.87) % while K (10.74), Ca (2.71), Mg (2.62), Zn (2.20), Na (2.08), Fe (3.57) and Cu (0.20) ppm were recorded in un-inoculated group of citrus plants that reduced to 3.15 and 0.76% and 3.66, 1.40, 0.63,0.42, 0.74, 1.13 and 0.13 ppm in inoculated group respectively. It was accomplished that susceptible varieties contained lower ionic contents than resistant varieties. The higher concentrations of ionic contents in resistant citrus varieties build up the biochemical and physiological processes of the citrus plant, which help to restrict spread of pathogens. Further research could explore the interplay between mineral nutrition and disease resistance in citrus, potentially leading to the development of new disease-resistant varieties.