Abstract：
Licania rigida Benth., a Brazilian endemic plant, has been traditionally used for treating inflammation and stomach pain. This work investigates the anti-inflammatory and gastroprotective activities of the ethanolic extract from L. rigida seeds (EELr) by in vitro and in vivo methods. The phytochemical profile was determined and the in vitro antioxidant activity was investigated by radical scavenging and thiobarbituric acid reactive substances methods. The ovalbumin denaturation method was used with sodium diclofenac as standard for the in vitro anti-inflammatory activity assessment. Acetylsalicylic acid was used to induce gastric ulcers in male mice and then to evaluate the preventive and therapeutic gastroprotective effect of EELr, using omeprazole as the reference drug. The extract exhibited relevant amount of phenolic compounds and flavonoids, in particular, demonstrating in vitro antioxidant capacity. EELr was able to inhibit almost 60% of ovalbumin denaturation at a concentration considered low. It also prevented the decrease of biochemical markers for oxidative stress such as superoxide dismutase (SOD) and reduced glutathione (GSH) in the stomach and SOD and catalase (CAT) in the liver. EELr also significantly decreased the number of lesions as well as reduced the ulcerated area when used as therapy. The observed effect may be due to its phenolic compounds, such as chlorogenic acid, caffeic acid and tannins, as previously reported. EELr is a potential source of compounds with anti-inflammatory activity, protects the liver from oxidative damage and improves healing of aspirin-induced ulcers. This work contributes to the knowledge of L. rigida species.
摘要：
LicaniarigdaBenth.,一种巴西特有植物，传统上用于治疗炎症和胃痛。这项工作通过体外和体内方法研究了来自L.rigda种子（EELr）的乙醇提取物的抗炎和胃保护活性。确定了植物化学特征，并通过自由基清除和硫代巴比妥酸反应性物质方法研究了体外抗氧化活性。卵清蛋白变性方法以双氯芬酸钠作为体外抗炎活性评估的标准。乙酰水杨酸用于诱导雄性小鼠胃溃疡，然后评估EELr的预防和治疗胃保护作用。使用奥美拉唑作为参考药物。提取物显示出相关量的酚类化合物和类黄酮，特别是,显示体外抗氧化能力。EELr在被认为是低的浓度下能够抑制几乎60%的卵清蛋白变性。它还可以防止氧化应激的生化标志物的减少，例如胃中的超氧化物歧化酶（SOD）和还原型谷胱甘肽（GSH）以及肝脏中的SOD和过氧化氢酶（CAT）。当用作治疗时，EELr还显着减少了病变的数量并减少了溃疡面积。观察到的效果可能是由于其酚类化合物，如绿原酸，咖啡酸和单宁，如先前报道。EELr是具有抗炎活性的化合物的潜在来源，保护肝脏免受氧化损伤，并改善阿司匹林引起的溃疡的愈合。这项工作有助于了解L.rigda物种。
