PDF(Pubmed)
Abstract：
Global antimicrobial resistance is a health crisis that can change the face of modern medicine. Exploring diverse natural habitats for bacterially-derived novel antimicrobial compounds has historically been a successful strategy. The deep-sea presents an exciting opportunity for the cultivation of taxonomically novel organisms and exploring potentially chemically novel spaces. In this study, the draft genomes of 12 bacteria previously isolated from the deep-sea sponges Phenomena carpenteri and Hertwigia sp. are investigated for the diversity of specialized secondary metabolites. In addition, early data support the production of antibacterial inhibitory substances produced from a number of these strains, including activity against clinically relevant pathogens Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Draft whole-genomes are presented of 12 deep-sea isolates, which include four potentially novel strains: Psychrobacter sp. PP-21, Streptomyces sp. DK15, Dietzia sp. PP-33, and Micrococcus sp. M4NT. Across the 12 draft genomes, 138 biosynthetic gene clusters were detected, of which over half displayed less than 50% similarity to known BGCs, suggesting that these genomes present an exciting opportunity to elucidate novel secondary metabolites. Exploring bacterial isolates belonging to the phylum Actinomycetota, Pseudomonadota, and Bacillota from understudied deep-sea sponges provided opportunities to search for new chemical diversity of interest to those working in antibiotic discovery.
摘要：
全球抗菌素耐药性是一场健康危机，可以改变现代医学的面貌。历史上，探索细菌衍生的新型抗菌化合物的多种自然栖息地是一种成功的策略。深海为培育分类学上新颖的生物和探索潜在的化学新颖空间提供了令人兴奋的机会。在这项研究中,先前从深海海绵Phenomenacarpenteri和Hertwigiasp.中分离出的12种细菌的基因组草案。研究了特殊次生代谢物的多样性。此外,早期数据支持由许多这些菌株产生的抗菌抑制物质的生产，包括针对临床相关病原体鲍曼不动杆菌的活性，大肠杆菌,肺炎克雷伯菌,铜绿假单胞菌,和金黄色葡萄球菌。提出了12个深海分离株的全基因组草案，其中包括四种潜在的新菌株：嗜冷杆菌。PP-21，链霉菌属。DK15,Dietziasp.PP-33和微球菌。M4NT.在12个基因组草案中，检测到138个生物合成基因簇,其中一半以上与已知的BGC相似度不到50%，这表明这些基因组为阐明新的次级代谢产物提供了令人兴奋的机会。探索属于放线菌门的细菌分离株，Pseudomonadota,和芽孢杆菌来自研究不足的深海海绵提供了机会，寻找新的化学多样性感兴趣的工作在抗生素的发现。
