源自海洋微生物的颜料和其他次生代谢产物已成为多方面应用的有前途的天然着色剂和药物。然而,产生这种天然分子的海洋放线菌在分类学方面的研究最少,生物医学中的化学多样性和应用,纺织品,和食品工业。在这项研究中,产生黄色素的革兰氏阳性放线菌,小单孢菌。菌株SH-82是从海洋海绵中分离出来的,Scopalinahapalia,并对其整个基因组进行了分析。菌株SH-82是多种化学分子的多产生产者,因为它在不同的培养条件下在A1培养基上产生更多的化合物。SH-82的基因组大小为6.24Mb(6,246,890bp),携带23个鉴定的生物合成基因簇。共有5415个CDS,60tRNA,9rRNA,从SH-82基因组中鉴定出1个tmRNA。全基因组的GC含量(%)为71.6%。菌株SH-82具有编码I型的基因,II型,和III型聚酮化合物合酶。基于多位点序列分析和脂肪酸甲酯(FAME)组成,菌株SH-82被证实为新物种。小单孢菌的遗传信息。SH-82已在BioProjectIDPRJNA1087320下保存到NCBI,序列读取存档(SRA)中的相应标识符为SAMN40439676,基因组登录名为CP148049。
Pigments and other secondary metabolites originating from marine microbes have been a promising natural colorants and drugs for multifaceted applications. However, marine actinobacteria producing such natural molecules are least investigated in terms of their taxonomy, chemical diversity and applications in biomedical, textile, and food industries. In this study, sioxanthin pigment-producing Gram-positive actinobacteria, Micromonospora sp. strain SH-82 was isolated from a marine sponge, Scopalina hapalia, and its whole genome was analyzed. Strain SH-82is a prolific producer of diverse chemical molecules as it produced more compounds on A1 medium with different culture conditions. The genome size of SH-82 is 6.24 Mb (6,246,890 bp) carrying 23 identified biosynthetic gene clusters. A total of 5415 CDS, 60 tRNA, 9 rRNA, and 1 tmRNA are identified from SH-82 genome. The GC content (%) of whole genome was 71.6%. Strain SH-82 harbors genes encoding type I, type II, and type III polyketide synthases. Based on the multi-locus sequence analysis and fatty acid methyl ester (FAME) composition, strain SH-82 is confirmed as a novel species. The genetic information of Micromonospora sp. SH-82 has been deposited to NCBI under the BioProject ID PRJNA1087320, with corresponding identifiers in the Sequence Read Archive (SRA) as SAMN40439676 and the Genome accession as CP148049.