Abstract：
This study investigated the possibility of using ethylenediaminetetraacetic acid functionalized silica nanoparticles (EDTA-SiO2) as a dentin-conditioning agent using etch-and-rinse technique to promote the durability of dentin bonding.
The SiO2-EDTA were synthesized by N- [(3- trimethoxysilyl) propyl] ethylenediamine triacetic acid (EDTA-TMS) and SiO2 (50 nm), then characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). The capacity of SiO2-EDTA to chelate calcium ions from dentin was examined by inductively coupled plasma-optic emission spectrometry (ICP-OES). The dentin surfaces conditioned with SiO2-EDTA were detected by field emission scanning electron microscopy (SEM), TEM and microhardness testing. For dentin bonding, dentin surfaces were adopted wet- or dry-bonding technique and bonded with adhesive (AdperTM Single Bond2) and applied composite resin (Filtek Z350) on them. The durability of dentin bonding was evaluated by mircotensile bond strength test, in-situ zymography and nanoleakage testing.
FTIR, TGA and XPS results showed that SiO2-EDTA contained N element and carboxyl groups. SEM, TEM and microhardness results indicated that SiO2-EDTA group created extrafibrillar demineralization and retained more intrafibrillar minerals within dentin surface. In the dentin bonding experiment, SiO2-EDTA group achieved acceptable bond strength, and reduced the activity of matrix metalloproteinase and nanoleakage along bonding interface.
It was possible to generate a feasible dentin conditioning agent (SiO2-EDTA), which could create dentin extrafibrillar demineralization and improve dentin bond durability.
This study introduces a new dentin conditioning scheme based on SiO2-EDTA to create extrafibrillar demineralization for dentin bonding. This strategy has the potential to be used in clinic to promote the life of restoration bonding.
The SiO2-EDTA were synthesized by N- [(3- trimethoxysilyl) propyl] ethylenediamine triacetic acid (EDTA-TMS) and SiO2 (50 nm), then characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). The capacity of SiO2-EDTA to chelate calcium ions from dentin was examined by inductively coupled plasma-optic emission spectrometry (ICP-OES). The dentin surfaces conditioned with SiO2-EDTA were detected by field emission scanning electron microscopy (SEM), TEM and microhardness testing. For dentin bonding, dentin surfaces were adopted wet- or dry-bonding technique and bonded with adhesive (AdperTM Single Bond2) and applied composite resin (Filtek Z350) on them. The durability of dentin bonding was evaluated by mircotensile bond strength test, in-situ zymography and nanoleakage testing.
FTIR, TGA and XPS results showed that SiO2-EDTA contained N element and carboxyl groups. SEM, TEM and microhardness results indicated that SiO2-EDTA group created extrafibrillar demineralization and retained more intrafibrillar minerals within dentin surface. In the dentin bonding experiment, SiO2-EDTA group achieved acceptable bond strength, and reduced the activity of matrix metalloproteinase and nanoleakage along bonding interface.
It was possible to generate a feasible dentin conditioning agent (SiO2-EDTA), which could create dentin extrafibrillar demineralization and improve dentin bond durability.
This study introduces a new dentin conditioning scheme based on SiO2-EDTA to create extrafibrillar demineralization for dentin bonding. This strategy has the potential to be used in clinic to promote the life of restoration bonding.
摘要：
目的：这项研究调查了使用乙二胺四乙酸功能化的二氧化硅纳米颗粒（EDTA-SiO2）作为牙本质调理剂的可能性，使用蚀刻和冲洗技术来促进牙本质结合的耐久性。
方法：由N-[（3-三甲氧基甲硅烷基）丙基]乙二胺三乙酸（EDTA-TMS）和SiO2（50nm）合成SiO2-EDTA，然后通过傅里叶变换红外光谱(FTIR)进行表征,热重分析（TGA），X射线光电子能谱(XPS)和透射电子显微镜(TEM)。通过电感耦合等离子体发射光谱法（ICP-OES）检查了SiO2-EDTA螯合牙本质中钙离子的能力。通过场发射扫描电子显微镜（SEM）检测用SiO2-EDTA处理的牙本质表面，TEM和显微硬度测试。对于牙本质粘合，牙本质表面采用湿粘合或干粘合技术，并用粘合剂（AdperTMSingleBond2）粘合，并在其上施加复合树脂（FiltekZ350）。通过mircotensile粘结强度测试评估了牙本质粘结的耐久性，原位酶谱和纳米泄漏测试。
结果：FTIR，TGA和XPS结果表明，SiO2-EDTA含有N元素和羧基。SEM,TEM和显微硬度结果表明，SiO2-EDTA组产生了纤丝外脱矿质，并在牙本质表面保留了更多的纤丝内矿物质。在牙本质结合实验中，SiO2-EDTA基团达到可接受的粘结强度，并降低了基质金属蛋白酶的活性和沿着键合界面的纳米孔隙。
结论：可以生成可行的牙本质调理剂（SiO2-EDTA），这可以产生牙本质纤维外脱矿质并提高牙本质粘结耐久性。
结论：本研究介绍了一种新的基于SiO2-EDTA的牙本质调理方案，以产生用于牙本质结合的纤维外脱矿质。该策略有可能在临床上用于促进修复结合的寿命。
方法：由N-[（3-三甲氧基甲硅烷基）丙基]乙二胺三乙酸（EDTA-TMS）和SiO2（50nm）合成SiO2-EDTA，然后通过傅里叶变换红外光谱(FTIR)进行表征,热重分析（TGA），X射线光电子能谱(XPS)和透射电子显微镜(TEM)。通过电感耦合等离子体发射光谱法（ICP-OES）检查了SiO2-EDTA螯合牙本质中钙离子的能力。通过场发射扫描电子显微镜（SEM）检测用SiO2-EDTA处理的牙本质表面，TEM和显微硬度测试。对于牙本质粘合，牙本质表面采用湿粘合或干粘合技术，并用粘合剂（AdperTMSingleBond2）粘合，并在其上施加复合树脂（FiltekZ350）。通过mircotensile粘结强度测试评估了牙本质粘结的耐久性，原位酶谱和纳米泄漏测试。
结果：FTIR，TGA和XPS结果表明，SiO2-EDTA含有N元素和羧基。SEM,TEM和显微硬度结果表明，SiO2-EDTA组产生了纤丝外脱矿质，并在牙本质表面保留了更多的纤丝内矿物质。在牙本质结合实验中，SiO2-EDTA基团达到可接受的粘结强度，并降低了基质金属蛋白酶的活性和沿着键合界面的纳米孔隙。
结论：可以生成可行的牙本质调理剂（SiO2-EDTA），这可以产生牙本质纤维外脱矿质并提高牙本质粘结耐久性。
结论：本研究介绍了一种新的基于SiO2-EDTA的牙本质调理方案，以产生用于牙本质结合的纤维外脱矿质。该策略有可能在临床上用于促进修复结合的寿命。
