Abstract：
Teucrium persicum Boiss. an Iranian endemic plant is used in Iranian traditional medicine. E-cadherin transmembrane protein participates in adherens junctions and is the main partner for β-catenin protein. The GC-MS analysis was used to detect the chemical constituents of the methanolic extract. Its effects on the transcription of the E-cadherin encoding gene, cellular levels, and localization of E-cadherin protein in PC-3 cells were investigated. About 70 chemical constituents were identified. Indirect immunofluorescence microscopy and western blotting results revealed the restoration of E-cadherin protein at cell adhesion contact sites in cells treated with T. persicum extract. Gene expression studies revealed that the extract increased the transcription of the E-cadherin encoding gene in PC-3 cells. These results suggest that T. persicum extract may contain potent compounds that provide further support for the anticancer properties of T. persicum. Surely, detailed molecular investigations are needed to find the mechanism(s) behind these effects.
摘要：
紫杉。一种伊朗特有植物被用于伊朗传统医学。E-cadherin跨膜蛋白参与粘附连接，是β-catenin蛋白的主要伴侣。GC-MS分析用于检测甲醇提取物的化学成分。其对E-cadherin编码基因转录的影响,细胞水平，并研究了E-cadherin蛋白在PC-3细胞中的定位。鉴定出大约70种化学成分。间接免疫荧光显微镜和蛋白质印迹结果显示，在用桃树提取物处理的细胞中，E-cadherin蛋白在细胞粘附接触部位恢复。基因表达研究表明，提取物增加了PC-3细胞中E-cadherin编码基因的转录。这些结果表明,桃子的提取物可能含有为桃子的抗癌性质提供进一步支持的有效化合物。当然,需要详细的分子研究来发现这些影响背后的机制。
