未经授权:紫苏是一种伊朗特有植物,用于伊朗传统医学。
未经批准:总酚和总黄酮含量,并测定了桃子甲醇提取物的抗氧化潜力。MTT法评价提取物对A-375细胞活力的抑制作用。克隆的,微核形成,采用吖啶橙/溴化乙锭染色法评价A-375细胞的存活和增殖。通过使用DNA片段化测定和测量半胱天冬酶3/7的活性来评估细胞凋亡。为了研究提取物对A-375细胞迁移的影响,采用体外伤口愈合(划痕)测定。
未经鉴定:提取物的平均总酚和类黄酮含量和抗氧化性能为6.97±0.011mg鞣花酸(EGA)/g,46.83±0.0019mg乙氧基喹(1,2-二氢-6-乙氧基-2,2,4-三甲基喹啉;EQ)/g干燥提取物,和10±0.002μg/ml,分别。毛竹甲醇提取物48小时的IC50值为13μg/ml。DNA片段化模式和caspase3/7的活性表明细胞活力的降低可能是由于凋亡诱导。微观观察显示核凝聚,微核形成的显著增加,和抑制用7μg/ml至15μg/ml的提取物处理的A-375细胞中的集落形成。伤口愈合试验支持提取物的抗迁移活性。
未经评估:T.仙子具有显著的抗氧化和细胞毒性。当然,需要更详细的分子和生化研究来发现这些影响背后的机制。
UNASSIGNED: Teucrium persicum is an Iranian endemic plant used in Iranian traditional medicine.
UNASSIGNED: The total phenolic and total flavonoid contents, and antioxidant potential of the methanolic extract of T. persicum were determined. The MTT test was used to evaluate the inhibitory effect of the extract on the viability of A-375 cells. The clonogenic, micronucleus formation, and acridine orange/ethidium bromide staining methods were used to evaluate the survival and proliferation of A-375 cells. Apoptosis was evaluated by using DNA fragmentation assay and measuring the activity of caspase 3/7. To study the effect of the extract on the migration of A-375 cells, the in vitro wound-healing (scratch) assay was employed.
UNASSIGNED: The average total phenolic and flavonoid contents and antioxidant properties of the extract were 6.97±0.011 mg Ellagic acid (EGA)/g, 46.83±0.0019 mg of the ethoxyquin (1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline; EQ)/g of dried extract, and 10±0.002 μg/ml, respectively. The IC50 value of the T. persicum methanolic extract was 13 μg/ml for 48 hr. The DNA fragmentation pattern and the activity of caspase3/7 suggested that the reduction of the cell viability may be due to apoptosis induction. Microscopic observations showed nuclear condensation, a considerable increase in micronuclei formation, and inhibition of the colony formation in A-375 cells treated with 7 μg/ml to 15 μg/ml of the extract. Wound-healing assay supported the anti-migration activity of the extract.
UNASSIGNED: T. persicum has significant antioxidant and cytotoxic properties. Surely, more detailed molecular and biochemical studies are needed to find the mechanism(s) behind these effects.