多年来,据推测,睾酮水平升高可能与前列腺癌的发生和增殖密切相关.
方法:体外研究了睾酮对激素非依赖性[PC-3]和激素依赖性[LNCAP]癌细胞代谢活性的影响。此外,通过流式细胞术获得睾酮纳米乳液[nanocare®]对细胞活力的影响。
结果:尽管正常前列腺和大多数前列腺癌对雄激素有依赖性,获得的结果表明,与普遍观点相反,在纳米乳剂中补充高剂量的睾酮能够降低前列腺癌细胞的代谢活性和活力。
结论:我们得出的结论是,在体外暴露于生物相合的睾酮的纳米乳液会降低激素非依赖性和激素依赖性前列腺癌细胞的生长。据我们所知,这是首次显示睾酮纳米乳对前列腺癌细胞代谢活性的潜在影响。这些测试表明,激素依赖性和激素依赖性前列腺癌细胞的生长被生物相同的睾酮的给药减少,这可能是前列腺癌诊断患者治疗的一个有趣的策略。
For many years, it has been speculated that elevated testosterone levels may be critically involved in the genesis and proliferation of prostate cancer.
METHODS: The effect of testosterone on the metabolic activity of hormone-independent [PC-3] and hormone-dependent [LNCAP] cancer cells was investigated in vitro. Additionally, the impact of testosterone nanoemulsion [nanocare®] on cell viability was accessed by flow cytometry.
RESULTS: Despite the dependency of the normal prostate and of most prostatic cancers upon androgens, the obtained results indicate that, contrary to prevailing opinion, the supplementation of testosterone with higher doses in nanoemulsion was able to lower the metabolic activity and viability of prostate cancer cells.
CONCLUSIONS: We conclude that the growth of hormone-independent and hormone-dependent prostate cancer cells was reduced by the exposure of a nanoemulsion of bioidentical testostosterone in vitro. To the best of our knowledge, this is the first time that the potential effect of a testosterone nanoemulsion on the metabolic activity of prostate cancer cells has been shown. Such tests suggest that the growth of hormone-independent and hormone-dependent prostate cancer cells was reduced by the administration of bioidentical testostosterone, and this might be an interesting strategy for prostate cancer treatment in diagnosed patients.