PDF(Pubmed)
Abstract：
Parvoviruses are small, single-stranded DNA viruses with non-enveloped capsids. Determining the capsid structures provides a framework for annotating regions important to the viral life cycle. Aleutian mink disease virus (AMDV), a pathogen in minks, and human parvovirus 4 (PARV4), infecting humans, are parvoviruses belonging to the genera Amdoparvovirus and Tetraparvovirus, respectively. While Aleutian mink disease caused by AMDV is a major threat to mink farming, no clear clinical manifestations have been established following infection with PARV4 in humans. Here, the capsid structures of AMDV and PARV4 were determined via cryo-electron microscopy at 2.37 and 3.12 Å resolutions, respectively. Despite low amino acid sequence identities (10-30%) both viruses share the icosahedral nature of parvovirus capsids, with 60 viral proteins (VPs) assembling the capsid via two-, three-, and five-fold symmetry VP-related interactions, but display major structural variabilities in the surface loops when the capsid structures are superposed onto other parvoviruses. The capsid structures of AMDV and PARV4 will add to current knowledge of the structural platform for parvoviruses and permit future functional annotation of these viruses, which will help in understanding their infection mechanisms at a molecular level for the development of diagnostics and therapeutics.
摘要：
细小病毒很小，具有无包膜衣壳的单链DNA病毒。确定衣壳结构提供了用于注释对病毒生命周期重要的区域的框架。阿留申水貂病病毒(AMDV),水貂身上的病原体,和人类细小病毒4（PARV4），感染人类,是一种细小病毒，属于双足病毒和四足病毒属，分别。虽然由AMDV引起的阿留申水貂病是水貂养殖的主要威胁，人类感染PARV4后尚未确定明确的临床表现.这里,AMDV和PARV4的衣壳结构通过低温电子显微镜在2.37和3.12μ分辨率下确定，分别。尽管氨基酸序列同一性低（10-30％），两种病毒都具有细小病毒衣壳的二十面体性质，有60种病毒蛋白（VPs）通过两种方式组装衣壳，三-，和五重对称VP相关的相互作用，但是当衣壳结构叠加到其他细小病毒上时，表面环中显示出主要的结构变异性。AMDV和PARV4的衣壳结构将增加细小病毒结构平台的现有知识，并允许这些病毒的未来功能注释，这将有助于在分子水平上了解它们的感染机制，以开发诊断和治疗方法。
