PDF(Pubmed)
Abstract：
Omphalotus japonicus is a major toxic mushroom in Japan. When food poisoning caused by O. japonicus occurs, quick and accurate identification using a method that does not rely on morphological discrimination is required. Because the loop-mediated isothermal amplification (LAMP) method meets these requirements, we developed a LAMP method for detecting O. japonicus. Amplification occurred within 60 min, and the presence or absence of O. japonicus was confirmed within 2 h, including the DNA extraction protocol. The LAMP method did not show cross-reactivity with 13 species of edible mushrooms, had high specificity toward O. japonicus, and had sufficient detection sensitivity even in a mixed mushroom sample containing 1% O. japonicus. Additionally, O. japonicus could be detected in simulated food poisoning samples of heated and digested mushrooms, and in actual food poisoning residual samples.
摘要：
日本的一种主要的毒蘑菇。当发生刺槐引起的食物中毒时，需要使用不依赖于形态辨别的方法进行快速准确的识别。因为环介导等温扩增（LAMP）方法满足这些要求，我们开发了一种LAMP方法来检测刺槐。扩增发生在60分钟内，并且在2小时内确认了刺槐的存在或不存在，包括DNA提取方案.LAMP方法未显示与13种食用蘑菇的交叉反应性，对刺槐有很高的特异性，即使在含有1％O.japonicus的混合蘑菇样品中，也具有足够的检测灵敏度。此外,在加热和消化的蘑菇的模拟食物中毒样品中可以检测到刺槐，以及实际食物中毒残留样本。
