%0 Journal Article
%T Loop-mediated isothermal amplification (LAMP) for rapid and easy identification of Omphalotus japonicus.
%A Sugano Y
%A Sakata K
%A Nakamura K
%A Hosokawa A
%A Kouguchi H
%A Suzuki T
%A Kondo K
%J Food Chem (Oxf)
%V 5
%N 0
%D Dec 2022 30
%M 35800296
暂无%R 10.1016/j.fochms.2022.100115
%X Omphalotus japonicus is a major toxic mushroom in Japan. When food poisoning caused by O. japonicus occurs, quick and accurate identification using a method that does not rely on morphological discrimination is required. Because the loop-mediated isothermal amplification (LAMP) method meets these requirements, we developed a LAMP method for detecting O. japonicus. Amplification occurred within 60 min, and the presence or absence of O. japonicus was confirmed within 2 h, including the DNA extraction protocol. The LAMP method did not show cross-reactivity with 13 species of edible mushrooms, had high specificity toward O. japonicus, and had sufficient detection sensitivity even in a mixed mushroom sample containing 1% O. japonicus. Additionally, O. japonicus could be detected in simulated food poisoning samples of heated and digested mushrooms, and in actual food poisoning residual samples.