{Reference Type}: Journal Article
{Title}: Loop-mediated isothermal amplification (LAMP) for rapid and easy identification of Omphalotus japonicus.
{Author}: Sugano Y;Sakata K;Nakamura K;Hosokawa A;Kouguchi H;Suzuki T;Kondo K;
{Journal}: Food Chem (Oxf)
{Volume}: 5
{Issue}: 0
{Year}: Dec 2022 30
暂无{DOI}: 10.1016/j.fochms.2022.100115
{Abstract}: Omphalotus japonicus is a major toxic mushroom in Japan. When food poisoning caused by O. japonicus occurs, quick and accurate identification using a method that does not rely on morphological discrimination is required. Because the loop-mediated isothermal amplification (LAMP) method meets these requirements, we developed a LAMP method for detecting O. japonicus. Amplification occurred within 60 min, and the presence or absence of O. japonicus was confirmed within 2 h, including the DNA extraction protocol. The LAMP method did not show cross-reactivity with 13 species of edible mushrooms, had high specificity toward O. japonicus, and had sufficient detection sensitivity even in a mixed mushroom sample containing 1% O. japonicus. Additionally, O. japonicus could be detected in simulated food poisoning samples of heated and digested mushrooms, and in actual food poisoning residual samples.