Abstract：
Codonopsis pilosula (Franch.) Nannf. (CPN), mainly planted in the northwest region, is a traditional Chinese medicine/good health food for nourishing qi and promoting blood circulation. This study firstly evaluated the inhibitory effects of the CPN extraction (CPNE) on α-glucosidase in vitro and in vivo, and tentatively confirmed its chemical ingredients by employing UHPLC-Triple-TOF-MS/MS. The CPNE had strong inhibitory activities against mammalian α-glucosidase (sucrase and maltase) and yeast α-glycosidase with semi-inhibitory concentrations (IC50) of 0.241 mg mL-1, 0.326 mg mL-1 and 1.167 mg mL-1, respectively. In addition, the CPNE could significantly decrease the postprandial blood glucose (PBG) levels in the sucrose/maltose/starch tolerance assays of diabetic mice. Furthermore, a total of 29 compounds, including 3 alkaloids, 13 phenolic acids, 8 alcohol glycosides and 5 alkynosides, were assigned based on comparison with the standards and references, as well as the analysis of main fragments. These results demonstrated that CPN could be used as an adjuvant therapy or dietary supplements to effectively control the occurrence and development of diabetes.
摘要：
党参（法国。）南夫。(CPN)，主要种植在西北地区，是滋气活血的中药/健康食品。本研究首先评价了CPN提取物(CPNE)对α-葡萄糖苷酶的体内外抑制作用,并通过UHPLC-Triple-TOF-MS/MS初步确认了其化学成分。CPNE对哺乳动物α-葡萄糖苷酶（蔗糖酶和麦芽糖酶）和酵母α-葡萄糖苷酶具有很强的抑制活性，半抑制浓度（IC50）分别为0.241mgmL-1，0.326mgmL-1和1.167mgmL-1。此外,CPNE可以显着降低糖尿病小鼠的蔗糖/麦芽糖/淀粉耐受测定中的餐后血糖（PBG）水平。此外，共有29个化合物,包括3种生物碱，13酚酸，8醇苷和5醇苷，根据与标准和参考文献的比较进行分配，以及主要碎片的分析。这些结果表明，CPN可以作为辅助治疗或膳食补充剂，有效控制糖尿病的发生和发展。
