PDF(Sci-hub)
Abstract：
Recent infectious disease outbreaks, such as COVID-19 and Ebola, have highlighted the need for rapid and accurate diagnosis to initiate treatment and curb transmission. Successful diagnostic strategies critically depend on the efficiency of biological sampling and timely analysis. However, current diagnostic techniques are invasive/intrusive and present a severe bottleneck by requiring specialist equipment and trained personnel. Moreover, centralised test facilities are poorly accessible and the requirement to travel may increase disease transmission. Self-administrable, point-of-care (PoC) microneedle diagnostic devices could provide a viable solution to these problems. These miniature needle arrays can detect biomarkers in/from the skin in a minimally invasive manner to provide (near-) real-time diagnosis. Few microneedle devices have been developed specifically for infectious disease diagnosis, though similar technologies are well established in other fields and generally adaptable for infectious disease diagnosis. These include microneedles for biofluid extraction, microneedle sensors and analyte-capturing microneedles, or combinations thereof. Analyte sampling/detection from both blood and dermal interstitial fluid is possible. These technologies are in their early stages of development for infectious disease diagnostics, and there is a vast scope for further development. In this review, we discuss the utility and future outlook of these microneedle technologies in infectious disease diagnosis.
摘要：
最近的传染病爆发，如COVID-19和埃博拉病毒，强调了快速准确诊断以启动治疗和遏制传播的必要性。成功的诊断策略关键取决于生物采样和及时分析的效率。然而,当前的诊断技术是侵入性/侵入性的，并且由于需要专业设备和训练有素的人员而成为严重的瓶颈。此外，集中式测试设施难以接近，旅行的要求可能会增加疾病传播。自我管理,现场护理(PoC)微针诊断设备可以为这些问题提供可行的解决方案。这些微型针阵列可以以微创方式检测皮肤中/来自皮肤的生物标志物以提供(近)实时诊断。很少有微针装置专门用于传染病诊断，尽管类似的技术在其他领域已经很成熟，并且通常适用于传染病的诊断。这些包括用于生物流体提取的微针，微针传感器和分析物捕获微针，或其组合。可以从血液和皮肤间质液进行分析物采样/检测。这些技术正处于传染病诊断的早期发展阶段，还有很大的发展空间。在这次审查中,我们讨论了这些微针技术在传染病诊断中的实用性和未来前景。
