未经证实:有效诱导了耳斑点图,内耳的发育起源来自人类多能干细胞(hPSC),为耳部发育和感音神经性听力损失建模提供了一个强大的平台。然而,通过逐步分化方法,hPSC的耳谱系规范能力有限,因为成功的耳细胞分化的关键因素尚未被彻底研究。在这项研究中,我们开发了一种新的分化系统,涉及使用具有信号因子的三维(3D)漂浮培养物,通过hPSC的逐步分化产生耳细胞谱系。
未经证实:我们在二维(2D)单层培养下将hPSC分化为前位细胞。然后,我们在成纤维细胞生长因子(FGF)的控制下,将诱导的前位细胞转移到3D漂浮培养物中,骨形态发生蛋白(BMP),维甲酸(RA)和WNT信号通路。我们使用免疫细胞化学评估了诱导细胞的特征,定量PCR(qPCR),人口平均,和单细胞RNA-seq(RNA-seq)分析。我们进一步研究了通过定义的转录因子的过表达使耳祖细胞向毛细胞分化的方法。
UNASSIGNED:我们证明了hPSC衍生的前胎盘细胞在FGF2和RA的3D漂浮培养中获得了分化成后胎盘细胞的潜力。随后WNT信号的激活诱导耳胎盘细胞形成。通过单细胞RNA-seq(scRNA-seq)分析,我们在诱导的球体中鉴定出多个成簇的耳斑状细胞和耳囊标记阳性细胞.此外,诱导的耳细胞显示出通过转录因子ATOH1,POU4F3和GFI1的过表达产生毛细胞样细胞的潜力。
UNASSIGNED:我们证明了FGF2、RA和WNT信号传导在3D环境中对于来自hPSC的耳谱系细胞的体外分化的关键作用。诱导的耳细胞具有分化成具有立体睫状束和尖端链状结构的内耳毛细胞的能力。该方案将用于感音神经性听力损失和人类内耳发育的体外疾病建模,从而有助于药物筛选和基于干细胞的再生医学。
UNASSIGNED: Efficient induction of the otic placode, the developmental origin of the inner ear from human pluripotent stem cells (hPSCs), provides a robust platform for otic development and sensorineural hearing loss modelling. Nevertheless, there remains a limited capacity of otic lineage specification from hPSCs by stepwise differentiation methods, since the critical factors for successful otic cell differentiation have not been thoroughly investigated. In this study, we developed a novel differentiation system involving the use of a three-dimensional (3D) floating culture with signalling factors for generating otic cell lineages via stepwise differentiation of hPSCs.
UNASSIGNED: We differentiated hPSCs into preplacodal cells under a two-dimensional (2D) monolayer culture. Then, we transferred the induced preplacodal cells into a 3D floating culture under the control of the fibroblast growth factor (FGF), bone morphogenetic protein (BMP), retinoic acid (RA) and WNT signalling pathways. We evaluated the characteristics of the induced cells using immunocytochemistry, quantitative PCR (qPCR), population averaging, and single-cell RNA-seq (RNA-seq) analysis. We further investigated the methods for differentiating otic progenitors towards hair cells by overexpression of defined transcription factors.
UNASSIGNED: We demonstrated that hPSC-derived preplacodal cells acquired the potential to differentiate into posterior placodal cells in 3D floating culture with FGF2 and RA. Subsequent activation of WNT signalling induced otic placodal cell formation. By single-cell RNA-seq (scRNA-seq) analysis, we identified multiple clusters of otic placode- and otocyst marker-positive cells in the induced spheres. Moreover, the induced otic cells showed the potential to generate hair cell-like cells by overexpression of the transcription factors ATOH1, POU4F3 and GFI1.
UNASSIGNED: We demonstrated the critical role of FGF2, RA and WNT signalling in a 3D environment for the in vitro differentiation of otic lineage cells from hPSCs. The induced otic cells had the capacity to differentiate into inner ear hair cells with stereociliary bundles and tip link-like structures. The protocol will be useful for in vitro disease modelling of sensorineural hearing loss and human inner ear development and thus contribute to drug screening and stem cell-based regenerative medicine.