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Abstract：
The mechanism of action of ssODN-directed gene editing has been a topic of discussion within the field of CRISPR gene editing since its inception. Multiple comparable, but distinct, pathways have been discovered for DNA repair both with and without a repair template oligonucleotide. We have previously described the ExACT pathway for oligo-driven DNA repair, which consisted of a two-step DNA synthesis-driven repair catalyzed by the simultaneous binding of the repair oligonucleotide (ssODN) upstream and downstream of the double-strand break. In order to better elucidate the mechanism of ExACT-based repair, we have challenged the assumptions of the pathway with those outlines in other similar non-ssODN-based DNA repair mechanisms. This more comprehensive iteration of the ExACT pathway better described the many different ways where DNA repair can occur in the presence of a repair oligonucleotide after CRISPR cleavage, as well as how these previously distinct pathways can overlap and lead to even more unique repair outcomes.
摘要：
ssODN指导的基因编辑的作用机制自成立以来一直是CRISPR基因编辑领域内讨论的主题。多个可比，但截然不同，已经发现了有和没有修复模板寡核苷酸的DNA修复途径。我们之前已经描述了寡核苷酸驱动的DNA修复的Exact途径，由两步DNA合成驱动的修复组成，该修复由双链断裂上游和下游的修复寡核苷酸（ssODN）的同时结合催化。为了更好地阐明基于Exact的修复机制，在其他类似的基于非ssODN的DNA修复机制中,我们用这些概述对该通路的假设提出了挑战.ExACT途径的这种更全面的迭代更好地描述了在CRISPR切割后，在修复寡核苷酸的存在下可以发生DNA修复的许多不同方式。以及这些先前不同的途径如何重叠并导致更独特的修复结果。
