Abstract：
Aspidopterys obcordata var. obcordata, a medicinal plant endemic to China, is a narrowly distributed species and wild resources are extremely limited. To evaluate the genetic variability and degree of genetic divergence of A. obcordata var. obcordata, and to make rational scientific decisions on its harvest and germplasm conservation, we collected 122 samples from across nearly all of its distribution area and studied genetic diversity using inter-simple sequence repeats (ISSRs), sequence-related amplified polymorphisms (SRAPs), and a method combining the two techniques. The results revealed the high genetic diversity of A. obcordata var. obcordata, mainly due to its intra-population diversity, and the top two populations with the highest levels of intra-population diversity were ML and DH, individuals of which can serve as excellent germplasm candidates during the processing of germplasm screening and conservation. In general, the combining method was prior to the ISSR analyses and SRAP analyses results, except for a slight difference in the genetic structure of individual populations. Therefore, we suggest that a combination analysis of the two marker methods is ideal for evaluating the genetic diversity and genetic relationships of A. obcordata var. obcordata.
摘要：
夜蛾。obcordata,一种中国特有的药用植物，属物种分布狭窄，野生资源极为有限。目的：评价分贝的遗传变异性和遗传分歧程度。obcordata,并对其收获和种质保护做出合理的科学决策，我们从几乎所有的分布区域收集了122个样本，并使用简单序列重复（ISSR）研究了遗传多样性，序列相关扩增多态性（SRAP），和结合这两种技术的方法。结果揭示了A.obcordatavar具有较高的遗传多样性。obcordata,主要是由于其种群内的多样性，种群内多样性水平最高的前两个种群是ML和DH，在种质筛选和保存过程中，其个体可以作为优秀的种质候选物。总的来说,组合方法是在ISSR分析和SRAP分析结果之前，除了个别群体的遗传结构略有差异。因此,我们建议两种标记方法的组合分析对于评估A.obcordatavar的遗传多样性和遗传关系是理想的。obcordata。
