malpighiaemarginata(Malpighiaceae),俗称“acerola”,是一种原产于美洲的热带和亚热带水果。尽管它的维生素C含量很高,这赋予了它很高的抗氧化性能,可溶性膳食纤维,如多糖,也是acerola的丰富成分(干果的10%)。针叶树冷水溶性(ACWS)部分在体内和体外均具有抗疲劳和抗氧化作用。为了推断ACWS的进一步全身效应,这项研究旨在调查抗伤害感受,抗炎,ACWS在小鼠疼痛模型中的抗氧化作用。在福尔马林诱导的伤害感受中,ACWS(0.1、1和10mg/kg)仅减少炎症期,并且(10和30mg/kg)减弱了乙酸诱导的扭体和腹膜腔中的白细胞迁移。ACWS(10mg/kg)大大减少了足底注射角叉菜胶引起的机械性异常疼痛和爪水肿。在角叉菜胶诱导的炎症选择(4小时),ACWS显著降低髓过氧化物酶活性,TNF-α,IL-1β,和PGE2水平,并恢复IL-10水平。ACWS还通过降低脂质氢过氧化物含量表现出抗氧化特性,增加GSH水平,并恢复角叉菜胶模型和2,2-二苯基-1-吡啶酰肼(DPPH)清除试验中的超氧化物歧化酶和过氧化氢酶活性。总的来说,这些结果支持抗伤害感受,抗炎,ACWS的抗氧化作用,并揭示了治疗炎性疼痛病症的有希望的候选药物。
Malpighia emarginata (
Malpighiaceae), popularly known as \"acerola\", is a tropical and subtropical fruit native to the Americas. Despite its high vitamin C content, which gives it a high antioxidant property, soluble dietary fibers, such as polysaccharides, are also abundant constituents of acerola (10% of the dried fruit). The acerola cold-water soluble (ACWS) fraction presented anti-fatigue and antioxidant effects in vivo and in vitro. To infer further systemic effects of ACWS, this study aimed to investigate the antinociceptive, anti-inflammatory, and antioxidant effects of ACWS in murine models of pain. In formalin-induced nociception, ACWS (0.1, 1, and 10 mg/kg) reduced only the inflammatory phase, and also (10 and 30 mg/kg) attenuated the acetic acid-induced writhing and leukocyte migration in the peritoneal cavity. The mechanical allodynia and paw edema induced by intraplantar injection of carrageenan were greatly reduced by ACWS (10 mg/kg). At the inflammatory pick induced by carrageenan (4 h), ACWS significantly reduced myeloperoxidase activity, TNF-α, IL-1β, and PGE2 levels, and restored IL-10 levels. ACWS also exhibited antioxidant properties by decreasing lipid hydroperoxides content, increasing GSH levels, and restoring superoxide dismutase and catalase activities in the carrageenan model and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. Collectively, these results support the antinociceptive, anti-inflammatory, and antioxidant effects of ACWS and reveal a promising candidate for the treatment of inflammatory pain conditions.