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Abstract：
Biofilms in dental unit waterlines (DUWL) are a potentially significant source of contamination posing a significant health risk as these may come into contact with patients and dental staff during treatment. The aim of this study was to evaluate the microbiological quality of DUWL water treated by Biofilm-Removing-System® (BRS®) and Alpron®/Bilpron® disinfectant solutions for six years in a French university hospital. The microbiological quality of water supplied by 68 dental units-initially shock treated with BRS®, then continuously treated by Alpron® with sterile water during working days and Bilpron® during inactivity period, and combined with purging every morning and after each patient-was assessed biannually during six years for total culturable aerobic bacteria at 22 °C and 36 °C, Legionella sp., Pseudomonas aeruginosa, and total coliforms. A total of 628 samples were analyzed, 99.8% were compliant with extended microbiological levels, and we never detected pathogen bacteria like Legionella sp. and P. aeruginosa. Only one sample (0.2%) was noncompliant with the level of total culturable aerobic bacteria at 36 °C, which exceeded 140 colony forming units per mL. The protocol implemented in our university hospital gives excellent results and enables control of the microbiological quality of DUWL water in the long term.
摘要：
牙科单元水线(DUWL)中的生物膜是潜在的显著污染源,造成显著的健康风险,因为这些可能在治疗期间与患者和牙科工作人员接触。这项研究的目的是评估在法国大学医院中使用生物膜去除系统®（BRS®）和Alpron®/Bilpron®消毒剂溶液处理的DUWL水的微生物质量。由68个牙科单位提供的水的微生物质量-最初用BRS®进行冲击处理，然后在工作日用无菌水连续处理Alpron®，在不活动期间用Bilpron®处理，并结合每天早晨和每位患者之后的吹扫-在六年中每年两次评估22°C和36°C的总可培养需氧细菌，军团菌。,铜绿假单胞菌,和总的大肠杆菌。共分析了628个样本,99.8%符合扩展的微生物水平，我们从未检测到像军团菌这样的病原体。还有铜绿假单胞菌.只有一个样品（0.2％）不符合36°C下可培养的需氧菌总数的水平，超过140个菌落形成单位/mL。在我们大学医院实施的协议提供了出色的结果，并能够长期控制DUWL水的微生物质量。
