Using Methylation-Specific PCR to Study RB1 Promoter Hypermethylation.

使用甲基化特异性 PCR 研究 RB1 启动子超甲基化。

• 发表时间: 2018
• 来源期刊: Methods Mol Biol
• DOI: 10.1007/978-1-4939-7565-5_4
• PMID: 29468541
• 文章类型: Journal Article
• 作者列表: McCormick TM,Carvalho MDGDC

关键词: 5-Methylcytosine; CpG island; Gene promoter methylation; Methylation-specific PCR; RB1; Sodium bisulfite treatment; Tumor suppressor gene

Mesh: DNA Methylation; DNA, Neoplasm / analysis; Gene Expression Regulation, Neoplastic; Humans; Neoplasms / genetics; Polymerase Chain Reaction / methods; Promoter Regions, Genetic; Retinoblastoma Binding Proteins / genetics; Ubiquitin-Protein Ligases / genetics

Abstract

It has increasingly been considered crucial the understanding of DNA methylation of Tumor Suppressor Gene (TSG) promoters, such as that of retinoblastoma 1 gene (RB1), and its role during carcinogenesis. We present a detailed and optimized protocol of the methylation-specific PCR (MSP) technique to study RB1 gene promoter hypermethylation.

摘要

它越来越被认为是至关重要的DNA甲基化的肿瘤抑制基因（TSG）启动子的理解，如视网膜母细胞瘤1基因（RB1），以及它在致癌过程中的作用。我们提出了甲基化特异性PCR（MSP）技术的详细优化方案，以研究RB1基因启动子超甲基化。