它越来越被认为是至关重要的DNA甲基化的肿瘤抑制基因(TSG)启动子的理解,如视网膜母细胞瘤1基因(RB1),以及它在致癌过程中的作用。我们提出了甲基化特异性PCR(MSP)技术的详细优化方案,以研究RB1基因启动子超甲基化。
It has increasingly been considered crucial the understanding of DNA methylation of Tumor Suppressor Gene (TSG) promoters, such as that of retinoblastoma 1 gene (RB1), and its role during carcinogenesis. We present a detailed and optimized protocol of the methylation-specific PCR (MSP) technique to study RB1 gene promoter hypermethylation.